ENU mutagenesis to generate genetically modified rat models.
ENU mutagenesis to generate genetically modified rat models.
复制标题
ENU 诱变产生转基因大鼠模型。
作者：
R. van Boxtel;M. Gould;E. Cuppen;B. Smits
大鼠是生物医学研究中最受欢迎的模式生物之一，在将生理学和病理学与基因组联系起来方面非常有用。然而，对大鼠生殖系中特定基因进行遗传修饰的方法仍然相对较少。迄今为止，产生转基因大鼠的最有效方法是基于靶向选择的N-乙基-N-亚硝基脲（ENU）诱变技术。在这里，我们描述了大鼠模型生物中ENU突变和突变体检索的详细方案。
The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.
影响因子：
14.9
作者：
Nickerson, DA;Tobe, VO;Taylor, SL
通讯作者：
Taylor, SL