Dietary fructooligosaccharides induce immunoregulation of intestinal IgA secretion by murine Peyer's patch cells
Dietary fructooligosaccharides induce immunoregulation of intestinal IgA secretion by murine Peyer's patch cells
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DOI：
10.1271/bbb.67.758
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发表时间：
2003-04-01
影响因子：
1.6
通讯作者：
Nakamura, R
中科院分区：
文献类型：
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作者：
Hosono, A;Ozawa, A;Nakamura, R
益生菌补充剂可诱导宿主产生免疫反应，而膳食低聚果糖（FOS）可刺激特定肠道菌群的生长。在本研究中，我们研究了口服FOS的免疫学影响。将BALB/c小鼠口服给予0 - 7.5%的FOS，持续6周，并测量肠道黏膜免疫反应。在2.5% FOS组中，粪便IgA显著增加。派氏结（PP）细胞分泌IgA对FOS呈剂量依赖性上调，并且来自PP的CD4(+) T细胞产生干扰素 - γ和白细胞介素（IL）10呈剂量依赖性增加，在产生IL - 5和IL - 6方面有高反应。相反，FOS抑制血清IgG1。我们的研究结果表明，补充FOS可改变肠道菌群环境，导致肠道黏膜中CD4(+) PP细胞的IgA分泌上调，并抑制以2型辅助性T细胞（Th2）为主的全身性免疫反应。
Probiotic supplements induce immunological responses in the host, and dietary fructooligosaccharides (FOS) stimulate the growth of selected intestinal microflora. In this study we investigated the immunological influences of orally administrated FOS. BALB/c mice were oral administered 0-7.5% FOS for 6 weeks, and the intestinal mucosal immune responses were measured. In the 2.5%-FOS group, fecal IgA was significantly increased. IgA secretion by Peyer's patch (PP) cells was upregulated in a dose-dependent way in response to FOS and CD4(+) T cells from PP showed a dose-dependent increase in production of interferon-gamma and interleukin (IL) 10, and a high response in production of IL-5 and IL-6. In contrast, FOS suppressed serum IgG1. Our findings suggest that FOS supplementation changes the intestinal environment of microflora, and leads to upregulation of IgA secretion in CD4(+) PP cells in intestinal mucosa, and to suppression of the systemic immune response to type 2 helper T (Th2) dominant.