Role of the dermonecrotic toxin of Bordetella bronchiseptica in the pathogenesis of respiratory disease in swine
Role of the dermonecrotic toxin of Bordetella bronchiseptica in the pathogenesis of respiratory disease in swine
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DOI：
10.1128/iai.70.2.481-490.2002
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发表时间：
2002-02-01
影响因子：
3.1
通讯作者：
Kunkle, RA
中科院分区：
文献类型：
--
作者：
Brockmeier, SL;Register, KB;Kunkle, RA
支气管败血波氏杆菌是引起猪萎缩性鼻炎和肺炎的病原之一。它产生几种所谓的毒力因子，包括皮肤坏死毒素（DNT），这与病例中看到的鼻甲萎缩有关！萎缩性鼻炎这些实验的目的是澄清这种毒素在呼吸道疾病中的作用，通过比较猪的两个同基因的dnt突变体的致病性，他们的毒力DNT+亲本菌株。进行了两个单独的实验，每个实验使用一对亲本-亲本。一周龄的剖腹产，初乳剥夺猪鼻内接种亲株，dnt突变株，或磷酸盐缓冲盐水。每周进行鼻冲洗以监测鼻腔定植，接种后4周对猪实施安乐死以确定组织定植并检查呼吸道病理学。有证据表明，亲本菌株在上呼吸道的定殖略大于dnt突变株，但在下呼吸道的定殖不多。在给予亲本菌株的大多数猪中发现中度鼻甲萎缩和支气管肺炎，而在用dnt突变株攻击的猪中没有发现鼻甲萎缩或肺炎。因此，由B产生DNT。支气管炎在感染该微生物的猪中产生鼻甲萎缩和支气管扩张的病变是必要的。
Bordetella bronchiseptica is one of the etiologic agents causing atrophic rhinitis and pneumonia in swine. It produces several purported virulence factors, including the dermonecrotic toxin (DNT), which has been implicated in the turbinate atrophy seen in cases! of atrophic rhinitis. The purpose of these experiments was to clarify the role of this toxin in respiratory disease by comparing the pathogenicity in swine of two isogenic dnt mutants to their virulent DNT+ parent strains. Two separate experiments were performed, one with each of the mutant-parent pairs. One-week-old cesarean-derived, colostrum-deprived pigs were inoculated intranasally with the parent strain, the dnt mutant strain, or phosphate-buffered saline. Weekly nasal washes were performed to monitor colonization of the nasal cavity, and the pigs were euthanized 4 weeks after inoculation to determine colonization of tissues and to examine the respiratory tract for pathology. There was evidence that colonization of the upper respiratory, tract, but riot the lower respiratory tract, was slightly greater for the parent strains than for the dnt mutants. Moderate turbinate atrophy and bronchopneumonia were found in most pigs given the parent strains, while there was no turbinate atrophy or pneumonia in pigs challenged with the dnt mutant strains. Therefore, production of DNT by B. bronchiseptica. is necessary to produce the lesions of turbinate atrophy and bronchopneumonia in pigs infected with this organism.