In vitro effects of lactoferrin on lipopolysaccharide-induced proliferation, gene expression, and prostanoid production by bovine peripheral blood mononuclear cells
In vitro effects of lactoferrin on lipopolysaccharide-induced proliferation, gene expression, and prostanoid production by bovine peripheral blood mononuclear cells
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DOI：
10.2460/ajvr.69.9.1164
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发表时间：
2008-09-01
影响因子：
1
通讯作者：
Lakritz, Jeffrey
中科院分区：
文献类型：
--
作者：
Dawes, Maisie E.;Tyler, Jeff W.;Lakritz, Jeffrey
目的：探讨乳铁蛋白对脂多糖（LPS）诱导的牛外周血单个核细胞（PBMCs）增殖、炎症介质基因表达和前列腺素生成的影响。样本种群-从15头荷斯坦公牛犊牛中分离的pbmcs。操作步骤-采用差速离心分离PBMCs混合群体。在96孔板中进行增殖试验，设计允许在棋盘设计中添加乳铁蛋白（200 ng/mL）和不添加LPS （1 mu g/mL）。(3) h -胸腺嘧啶的掺入测定pbmc的增殖。用酶免疫法测定培养条件下前列腺素e2的产生。采用PCR检测乳铁蛋白对lps诱导的环氧合酶(COX)-2和基质金属蛋白酶(MMP)-9基因表达的影响。结果：补充乳铁蛋白可显著降低脂多糖诱导pbmc的3h -胸腺嘧啶掺入和前列腺素e2的产生。乳铁蛋白降低lps诱导的COX-2和MMP-9 mRNA的表达。结论及临床意义：乳铁蛋白在体外降低lps诱导的牛pbmc细胞增殖、炎症介质基因表达和前列腺素e2的产生。这些作用可能有利于减少新生儿内毒素血症的影响。
Objective-To evaluate the effect of lactoferrin on lipopolysaccharide (LPS)-induced proliferation of bovine peripheral blood mononuclear cells (PBMCs), gene expression of inflammatory mediators, and production of prostanoids in vitro.Sample Population-PBMCs isolated from 15 Holstein bull calves.Procedures-Mixed populations of PBMCs were isolated by differential centrifugation. Proliferation assays were conducted in 96-well plates designed to allow addition of lactoferrin (200 ng/mL) with and without LPS (1 mu g/mL) in a checkerboard design. Incorporation of (3)H-thymidine was used to determine proliferation of PBMCs. Prostaglandin E 2 production was determined in culture-conditioned medium by use of enzyme immunoassay. Effects of lactoferrin on LPS-induced gene expression of cyclooxygenase (COX)-2 and matrix metalloproteinase (MMP)-9 were monitored by use of PCR assays.Results-Lactoferrin supplementation significantly reduced LPS-induced incorporation of 3H-thymidine and production of prostaglandin E 2 by PBMCs. Lactoferrin reduced LPS-induced expression of COX-2 and MMP-9 mRNA.Conclusions and Clinical Relevance-Lactoferrin reduced LPS-induced cellular proliferation, inflammatory mediator gene expression, and prostaglandin E 2 production by bovine PBMCs in vitro. These effects may be beneficial in reducing the impact of endotoxemia in neonates.