PHOTOCHEMICAL ACTIVITY AND COMPONENTS OF MEMBRANE PREPARATIONS FROM BLUE-GREEN-ALGAE .1. COEXISTENCE OF 2 PHOTOSYSTEMS IN RELATION TO CHLOROPHYLL ALPHA AND REMOVAL OF PHYCOCYANIN
PHOTOCHEMICAL ACTIVITY AND COMPONENTS OF MEMBRANE PREPARATIONS FROM BLUE-GREEN-ALGAE .1. COEXISTENCE OF 2 PHOTOSYSTEMS IN RELATION TO CHLOROPHYLL ALPHA AND REMOVAL OF PHYCOCYANIN
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DOI：
10.1016/0005-2728(74)90063-2
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
WADA, K
中科院分区：
文献类型：
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作者：
ARNON, DI;MCSWAIN, BD;WADA, K
将念珠藻（Nostoc muscorum）（菌株7119）细胞破碎，并通过毛地黄皂苷处理从膜碎片中去除辅助色素藻蓝蛋白。藻蓝蛋白耗尽的膜片段保留了光系统I和光系统II的活性，这可以通过水或还原的2，6-dichlorophenolindophenol的NADP+光还原的高速率来证明，表明藻蓝蛋白不是电子传递活性的必需组分。即使是激烈的毛地黄皂苷处理未能显着减少显着稳定的电子从水到NADP+的传输。行动光谱和相对量子效率的测量表明，存在的光系统I和光系统II的膜片段，其中含有叶绿素作为唯一显着的光吸收色素。
Nostoc muscorum(Strain 7119) cells were disrupted and the accessory pigment phycocyanin was removed from membrane fragments by digitonin treatment. The phycocyanin-depleted membrane fragments retained both Photosystem I and Photosystem II activity, as evidenced by high rates of NADP+photoreduction either by water or by reduced 2,6-dichlorophenolindophenol, indicating that phycocyanin is not an essential component for electron transport activity.No separation of the two photosystems was effected by the digitonin treatment. Even drastic digitonin treatments failed to diminish significantly the remarkably stable electron transport from water to NADP+.Action spectra and relative quantum efficiency measurements demonstrated the existence of both Photosystem I and Photosystem II in membrane fragments which contained chlorophyllaas the only significant light-absorbing pigment.