Tetramethylpyrazine accelerates the function recovery of traumatic spinal cord in rat model by attenuating inflammation
Tetramethylpyrazine accelerates the function recovery of traumatic spinal cord in rat model by attenuating inflammation
复制标题
DOI：
10.1016/j.jns.2012.10.009
复制
发表时间：
2013-01-15
影响因子：
4.4
通讯作者：
Lu, Hong-Bin
中科院分区：
文献类型：
--
作者：
Hu, Jian-Zhong;Huang, Jiang-Hu;Lu, Hong-Bin
在本研究中，我们探讨了川芎嗪（TMP）（一种从中草药川芎中提取的生物碱）对脊髓挫伤（SCI）大鼠模型的影响。采用改良的Allen's减重法在大鼠中诱导挫伤性SCI模型，对T10节段的严重程度为5 g x 50 mm。在TMP治疗组中，大鼠腹膜内（i.p.）注射TMP（200 mg/kg），每24小时注射一次，持续5天，从挫伤SCI后半小时开始。对照组用生理盐水治疗。与对照组相比，TMP组明显改善大鼠后肢功能恢复。 TMP治疗显着降低巨噬细胞迁移抑制因子、核因子κB、促炎细胞因子白细胞介素18和中性粒细胞浸润的表达。另一方面，TMP 增强了抑制剂 kappa B 和抗炎细胞因子白细胞介素 10 的表达。总之，我们的结果表明，TMP 抑制大鼠脊髓挫伤相关炎症和组织损伤的发展，这可能会改善大鼠的后肢功能。 (C) 2012 Elsevier B.V. 保留所有权利。
In the present study, we explored the effects of tetramethylpyrazine (TMP), an alkaloid extracted from the Chinese herbal medicine Ligusticum wallichii Franchat (chuanxiong), on a rat model of contusion spinal cord injury (SCI). The contusion SCI model was induced in rats by a modified Allen's weight-drop method with a severity of 5 g x 50 mm impacting on the T10 segment In the TMP treatment group, rats were injected intraperitoneally (i.p.) with TMP (200 mg/kg), every 24 h for 5 days, starting half an hour after contusion SCI. The control group was treated with saline. Compared with the control group, the TMP group significantly ameliorated the recovery of hindlimb function of rats. TMP treatment significantly reduced the expression of macrophage migration inhibitory factor, nuclear factor kappa B, pro-inflammatory cytokine interleukin-18 and neutrophil infiltration. On the other hand, TMP enhanced the expression of inhibitor kappa B and anti-inflammation cytokine interleukin-10. In conclusion, our results demonstrate that TMP inhibits the development of inflammation and tissue injury associated with spinal cord contusion in rats which may improve the rats' hindlimb function. (C) 2012 Elsevier B.V. All rights reserved.