An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes
An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes
复制标题
DOI：
10.1016/j.jim.2011.08.013
复制
发表时间：
2011-10-28
影响因子：
2.2
通讯作者：
Kovacs, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Brubaker, Aleah L.;Schneider, David F.;Kovacs, Elizabeth J.
事实证明，从全层切除伤口中分离白细胞是一个困难的过程，会导致细胞产量低下，并对功能测定造成重大限制。考虑到对伤口衍生的细胞群的分离、表征和功能测量的兴趣增加，本文中我们描述了一种用于制备伤口细胞悬浮液的方法，其具有能够进行表型和功能评估的提高的产率。(C)2011 Elsevier B.V.保留所有权利。
Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments. (C) 2011 Elsevier B.V. All rights reserved.