Screening of selective lignin-degrading basidiomycetes and biological pretreatment for enzymatic hydrolysis of bamboo culms
Screening of selective lignin-degrading basidiomycetes and biological pretreatment for enzymatic hydrolysis of bamboo culms
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DOI：
10.1016/j.ibiod.2012.05.042
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发表时间：
2012-11-01
影响因子：
4.8
通讯作者：
Meguro, Sadatoshi
中科院分区：
文献类型：
--
作者：
Suhara, Hiroto;Kodama, Sayaka;Meguro, Sadatoshi
对51株真菌进行了筛选，筛选出适合毛竹酶法糖化预处理的菌株。这些真菌是从腐烂的竹秆内部或竹秆上的担子上分离的。处理12周后，白色腐烂的担子菌Punctularia sp. TUFC 20056和一种未鉴定的担子菌TUFC 20057显示出高木质素降解率（>50%）和高木质素/综纤维素损失率（>6）。其它斑点点霉属菌株也显示出高的木质素/综纤维素比率（>4）。在斑节孢TUFC 20056处理的竹子的残留成分中，葡萄糖和总糖分别增加了42.9 ± 10.9%和60.3 ± 16.1%。竹秆经点斑霉TUFC 20056预处理后，酶解效果明显提高。处理1个月总还原糖产量达10.1 ± 1.2%，高于两种著名的白色腐菌。斑点霉TUFC 20056可能适合于竹材的生物预处理，以生产生物乙醇。(c)2012爱思唯尔有限公司保留所有权利。
Fifty-one fungal isolates Were screened for their biological pretreatment of bamboo culms (Phyllostachys pubescence) by enzymatic saccharification. These fungi were isolated from the interior of decayed bamboo culms or from basidiomata on bamboo culms. The white rot basidiomycete Punctularia sp. TUFC20056 and an unidentified basidiomycete TUFC20057 showed high lignin degradation (>50%) and high lignin/holocellulose loss ratios (>6) after 12 weeks of treatment. Other Punctularia sp. strains also showed high lignin/holocellulose ratios (>4). In residual component of Punctularia sp. TUFC20056-treated bamboo, glucose and total sugar were increased (42.9 +/- 10.9 and 60.3 +/- 16.1%, respectively). Pretreatment of bamboo culms with Punctularia sp. TUFC20056 improved the effect of enzymatic hydrolysis. Total reducing sugar yields reached 10.1 +/- 1.2% with 1 month of treatment, and higher than that of two famous white rot fungi. Punctularia sp. TUFC20056 may be suitable for the biological pretreatment of bamboo for bioethanol production. (c) 2012 Elsevier Ltd. All rights reserved.