The origin of the supernumerary subunits and assembly factors of complex I: A treasure trove of pathway evolution
The origin of the supernumerary subunits and assembly factors of complex I: A treasure trove of pathway evolution
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DOI：
10.1016/j.bbabio.2016.03.027
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发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
Huynen, Martijn A.
中科院分区：
文献类型：
--
作者：
Elurbe, Dei M.;Huynen, Martijn A.
我们回顾和文件的进化起源的所有复合体I组装因子和9个多余的亚基从蛋白质家族。根据实验数据和关键残基的保守性，我们确定了络合物I代表物与其非络合物I同源物之间的蛋白质功能守恒谱。该光谱范围从保留其分子功能但底物特异性可能已经改变或变得更加特异性的蛋白质（如NDUFAF5）到失去其原始分子功能和关键催化残基的蛋白质（如NDUFAF6）。介于两者之间的是保留其分子功能的蛋白质，但似乎与复合物I无关，如ACAD9，或活性位点的氨基酸保守但没有酶活性的蛋白质，如NDUFA10。我们在分子进化理论的背景下解释复合体I的进化。复合体I多余的亚基和组装因子似乎是从线粒体和/或复合体I活跃时表达的蛋白质中招募来的。在复合体I及其组装的进化过程中，有许多基因复制后的新功能化案例，如ACAD9和TMEM126B；一个亚功能化案例：脂质耶氏菌中的ACPM1和ACPM2；一个复合体I蛋白本身似乎是另一个复合体新蛋白的来源：NDUFS6产生细胞色素c氧化酶亚基COX4/COX5b。因此，复合体I及其组装可以被视为途径进化的宝库。这篇文章是由Volker Zickermann和Ulrich Brandt编辑的题为呼吸复合体I的特刊的一部分。(C) 2016 Elsevier B.V.版权所有
We review and document the evolutionary origin of all complex I assembly factors and nine supernumerary subunits from protein families. Based on experimental data and the conservation of critical residues we identify a spectrum of protein function conservation between the complex I representatives and their non-complex I homologs. This spectrum ranges from proteins that have retained their molecular function but in which the substrate specificity may have changed or have become more specific, like NDUFAF5, to proteins that have lost their original molecular function and critical catalytic residues like NDUFAF6. In between are proteins that have retained their molecular function, which however appears unrelated to complex I, like ACAD9, or proteins in which amino acids of the active site are conserved but for which no enzymatic activity has been reported, like NDUFA10. We interpret complex I evolution against the background of molecular evolution theory. Complex I supernumerary subunits and assembly factors appear to have been recruited from proteins that are mitochondrial and/or that are expressed when complex I is active. Within the evolution of complex I and its assembly there are many cases of neofunctionalization after gene duplication, like ACAD9 and TMEM126B, one case of subfunctionalization: ACPM1 and ACPM2 in Yarrowia lipolytica, and one case in which a complex I protein itself appears to have been the source of a new protein from another complex: NDUFS6 gave rise to cytochrome c oxidase subunit COX4/COX5b. Complex I and its assembly can therewith be regarded as a treasure trove for pathway evolution. This article is part of a Special Issue entitled Respiratory complex I, edited by Volker Zickermann and Ulrich Brandt. (C) 2016 Elsevier B.V. All rights reserved.