Generalized recombination: Nucleotide sequence homology between chi recombinational hotspots
Generalized recombination: Nucleotide sequence homology between chi recombinational hotspots
复制标题
广义重组：chi 重组热点之间的核苷酸序列同源性
DOI：
10.1016/s0092-8674(80)80054-7
复制
发表时间：
1980
期刊：
影响因子：
64.5
通讯作者：
J. Crasemann
中科院分区：
文献类型：
--
作者：
Gerald R. Smith;D. Schultz;J. Crasemann
CHI位点刺激了由依赖于RecA-RecBC的大肠杆菌系统催化的广泛重组。这种刺激发生在CHI位点周围数千个碱基对的区域。这些位点是由噬菌体λ中四个不同位点的突变引起的。我们在这里报告了其中一个基因座χB的核苷酸序列，该基因座位于exis和redA基因之间。通过缺失或颠换成GC碱基对来改变单个CGb碱基对，产生CHI重组热点χ+B。在30bp的区段中，χ+B序列与先前确定的χ+C序列有23个碱基对相同。我们假设这个共同序列的某一部分是对蛋白质的识别序列，该序列作用于广义重组的限速步骤。
Chi sites stimulate generalized recombination catalyzed by the RecA-RecBC-dependent system of E. coli. This stimulation occurs over a region of several thousand base pairs surrounding the Chi site. These sites arise by mutation at four distinct loci in bacteriophage λ. We report here the nucleotide sequence surrounding one of these loci, χB, located between thexisandredAgenes. Alteration of a singleCGbase pair, by deletion or by transversion to aGCbase pair, creates the Chi recombinational hotspot χ+B. In a section of 30 bp, the χ+Bsequence has 23 bp in common with the χ+Csequence determined previously. We presume that some part of this common sequence is the recognition sequence for a protein which acts at a rate-limiting step of generalized recombination.