Degradation properties of chitosan microspheres/poly(L-lactic acid) composite in vitro and in vivo
Degradation properties of chitosan microspheres/poly(L-lactic acid) composite in vitro and in vivo
复制标题
壳聚糖微球/聚(L-乳酸)复合材料的体外和体内降解性能。
DOI：
10.1016/j.carbpol.2018.03.067
复制
发表时间：
2018-08-01
影响因子：
11.2
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Guo, Zhenzhao;Bo, Dongying;Li, Hong
聚L-乳酸（PLLA）是一种广泛应用的可生物降解高分子材料，但其降解过程中的累积产酸量是制约其广泛应用的瓶颈。在这项研究中，壳聚糖微球（CSM）被引入到PLLA多孔基质中，以操纵PLLA降解的酸性微气候。分别在PBS中进行体外和体内降解，并植入大鼠皮下。结果表明，CSMs/PLLA复合材料的pH值在体外保持稳定，约为7.50，直到6周。随着CSM含量的增加，复合材料的失重率、吸水率增加，PLLA的Mv减小。此外，通过H&E染色观察，CSM的引入可通过明显的形态学破坏加速CSM/PLLA复合材料的体内降解。这些结果表明，CSM的掺入不仅缓冲了酸性微气候，而且改善了PLLA的降解。本研究开发的CSM/PLLA复合材料降解行为可调，降解微气候温和，将为其生物医学应用提供有益的启示。
Poly(L-lactic acid) (PLLA) is a popular biodegradable polymer, but the accumulated acid production by degradation is a bottleneck for its extended utilization. In this study, chitosan microspheres (CSMs) were introduced into PLLA porous matrices to manipulate the acid microclimate from PLLA degradation. In vitro and in vivo degradation were, respectively, performed in PBS and implanted into rat's subcutaneous. The results indicated that pH of CSMs/PLLA composites remained stable of around 7.50 in vitro until six weeks. With the increasing of CSMs, mass loss, water absorption of the composites increased, while Mv of PLLA decreased during in vitro test. Furthermore, CSMs/PLLA composites degradation in vivo could be speeded by the introduction of CSMs via obvious morphological destroy under H&E staining observation. These results indicated that incorporation of CSMs not only buffered the acidic microclimate but also improved the degradation of PLLA. The tunable degradation behavior and moderate degradation microclimate of CSMs/PLLA composites developed in the study would provide benefits for its biomedical application.