Effect of colchicine on mitosis in the neural tube of the forty‐eight hour chick embryo
Effect of colchicine on mitosis in the neural tube of the forty‐eight hour chick embryo
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秋水仙碱对48小时鸡胚神经管有丝分裂的影响
DOI：
10.1002/ar.1090900108
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发表时间：
1944
期刊：
影响因子：
--
通讯作者：
S. Estes
中科院分区：
文献类型：
--
作者：
T. Woodard;S. Estes
秋水仙素作用于有丝分裂细胞核的OIW是产生中期阻滞。在适当的浓度下，随着时间的推移，更多的I ~ O I ~ C核受到影响，因此，在计数中。有丝分裂细胞核的数量似乎减少了。这种增加首先被许多人解释为意味着有丝分裂被刺激了。然后认识到，如果在给定时间内进行的所有有丝分裂在中期被阻止，则所有增加可能仅仅是该阶段细胞核积累的结果（Ludford，'36）。后一种解释现在被广泛接受，但即使如此，秋水仙素在动物材料上的某些结果也被归因于stiniulatiou。例如，在小鸡中就是这种情况，PafY（'39）发现，用α-olehicine处理48小时小鸡胚的一个结果是减少神经管中过度生长的局部区域。他将这些归因于有丝分裂刺激。因此，如果可能的话，希望以定量的方式确定秋水仙素是否刺激有丝分裂。鉴于秋水仙碱作为一种实验方法被频繁地用于阻止有丝分裂，这一点尤其重要。
OIW of t l i ~ d fwt s of colchicine upon mitotic nuclei is to produce inetaphase block. At adequate concentrations and as time goes 011 more aud I ~ O I ' C nuclei a re thus affected, so that in counts the number. of mitotic iiuclei appears to illcrease. This increase was first interpreted by many to mean that mitosis had been stimulated. It was then realized that if all mitosis in progress at a giveii time is arrested at the metaphase ail increase might be the result simply of an accumulation of nuclei in this phase (Ludford, '36) . The latter interpretation is now geiierdly accepted, but even yeceiitly certain results with colchicine on animal material have been attributed to stiniulatiou. This is the case, for example, in the chick i i i which PafY ( '39) found that one result of treating 48-hour' chick cnibryos with (.olehicine was to pi-oduee local regions of overgrowth in the neural tube. These he attributed to mitotic stimulation. It seeins desirable, therefore, to determine in a quantitative way, if possible, whether or not mitosis is stimulated by colchicine. This is especially important in view of the frequency with which colchicine is being used to arrest mitosis as an experimental method.