Monitoring DNA structures by dual fluorescence of pyrene derivatives
Monitoring DNA structures by dual fluorescence of pyrene derivatives
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DOI：
10.1021/ja053609e
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发表时间：
2005-09-28
影响因子：
15
通讯作者：
Saito, I
中科院分区：
文献类型：
--
作者：
Okamoto, A;Tainaka, K;Saito, I
我们开发了一种由芘衍生物修饰的具有双荧光的核苷酸。根据 DNA 结构状态，在环境温度下有效控制掺入 DNA 的荧光团的双荧光。我们的双荧光核苷作为概念上新的探针，可有效地通过颜色变化监测 DNA 杂交，而无需使用荧光染料进行多重标记。
We have developed a nucleotide modified by a pyrene derivative with dual fluorescence. The dual fluorescence of the fluorophore, which was incorporated into DNA, was effectively controlled at ambient temperature according to DNA structural status. Our nucleoside with dual fluorescence is effective as a conceptually new probe for monitoring DNA hybridization by the color change without multilabeling with fluorescent dyes.