Translocation of PpNPR1 is required for β-aminobutyric acid-triggered resistance against Rhizopus stolonifer in peach fruit
Translocation of PpNPR1 is required for β-aminobutyric acid-triggered resistance against Rhizopus stolonifer in peach fruit
复制标题
PpNPR1 的易位是桃果实中 β-氨基丁酸触发的对匍匐根霉的抗性所必需的
DOI：
10.1016/j.scienta.2020.109556
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发表时间：
2020-10-15
影响因子：
4.3
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
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作者：
Li, Chunhong;Wang, Kaituo;Zheng, Yonghua
本研究试图确定桃果实对 β-氨基丁酸 (BABA) 诱导和匍匐根霉接种的转录组反应，并确定关键差异表达基因 (DEG) 以及 BABA + 接种与对照和接种与对照配对比较之间显着富集的途径。此外，根据 BABA 处理样品中发病机制相关 (PR) 基因的表达，研究了关键 DEG 及其相互作用蛋白之间的调控联系。结果表明，转录因子PpTGA1在蛋白质-蛋白质相互作用（PPI）网络中具有最高程度，被确定为关键DEG。 PpTGA1可以通过水杨酸（SA）依赖性信号通路直接与细胞核中显着表达的PpNPR1基因相互作用，最终导致PpPR1和PpPR1-like的高转录水平。此外，在用 BABA 预处理和随后的 R. stolonifer 攻击的桃子中，氧化还原信号分子（一氧化氮 (NO) 和 SA）生成的增加伴随着对根霉腐烂的明显抑制作用。因此，BABA诱导和随后的R.stolonifer接种正向调节细胞间氧化还原状态，从而诱导高表达的PpNPR1基因的产物易位至细胞核，在细胞核中与PpTGA1相互作用，最终增强防御相关基因的表达和抗病性。
This study attempted to determine the transcriptomic responses of peach fruit to beta-aminobutyric acid (BABA) elicitation and Rhizopus stolonifer inoculation and to identify the key differentially expressed genes (DEGs) and significantly enriched pathways between BABA + inoculation-versus-control and inoculation-versus-control pairwise comparisons. Furthermore, the regulatory linkage between the critical DEGs and their interacting proteins was investigated based on the expression of pathogenesis-related (PR) genes in the BABA-treated samples. The results showed that the transcription factor PpTGA1 possessed the highest degree in the protein-protein interaction (PPI) network and was identified as a critical DEG. PpTGA1 could directly interact with the significantly expressed PpNPR1 gene in the nucleus through the salicylic acid (SA)-dependent signalling pathway, eventually leading to high transcript levels of PpPR1 and PpPR1-like. In addition, an increased generation of redox signalling molecules (nitric oxide (NO) and SA) was accompanied by an obvious effect on the suppression of Rhizopus rot in peaches pretreated with BABA and subsequent R. stolonifer challenge. Thus, BABA elicitation and subsequent R. stolonifer inoculation positively modulated the intercellular redox state and consequently induced the translocation of the product of the highly expressed PpNPR1 gene to the nucleus, where it interacts with PpTGA1 and eventually strengthens defence-related gene expression and disease resistance.