MALDI imaging MS of phospholipids in the mouse lung
MALDI imaging MS of phospholipids in the mouse lung
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DOI：
10.1194/jlr.m015750
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发表时间：
2011-08-01
影响因子：
6.5
通讯作者：
Murphy, Robert C.
中科院分区：
文献类型：
--
作者：
Berry, Karin A. Zemski;Li, Bilan;Murphy, Robert C.
脂质介质在肺生物化学中是重要的，并且源自花生四烯酸和二十二碳六烯酸的酶促氧化，花生四烯酸和二十二碳六烯酸是存在于细胞膜中的磷脂中的PUFA。在这项研究中，MALDI成像MS被用来确定本地化的花生四烯酸和二十二碳六烯酸含有磷脂在小鼠肺。这些含有PUFA的磷脂被确定为在小气道和大气道的衬里处是独特丰富的，这通过免疫组织化学明确地鉴定。此外，发现肺中存在的血管以鞘磷脂分子种类为特征，肺表面活性剂磷脂似乎均匀分布在整个肺实质中，表明肺泡定位。该技术揭示了肺组织中气道内含有出乎意料的高浓度花生四烯酸和二十二碳六烯酸磷脂，这可能是影响气道生物学的脂质介质的前体。贝里，K。A. Z.，B。Li，S. D.雷诺兹河M.巴克利，M. A.希洪，J. A. Hankin，P. M. Henson和R. C.墨菲小鼠肺中磷脂的MALDI成像MS。J. Lipid Res. 2011. 52：1551-1560。
Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are PUFAs that are present in phospholipids in cell membranes. In this study, MALDI imaging MS was used to determine the localization of arachidonate-and docosahexaenoate-containing phospholipids in mouse lung. These PUFA-containing phospholipids were determined to be uniquely abundant at the lining of small and large airways, which were unequivocally identified by immunohistochemistry. In addition, it was found that the blood vessels present in the lung were characterized by sphingomyelin molecular species, and lung surfactant phospholipids appeared evenly distributed throughout the lung parenchyma, indicating alveolar localization. This technique revealed unexpected high concentrations of arachidonate-and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology.-Berry, K. A. Z., B. Li, S. D. Reynolds, R. M. Barkley, M. A. Gijon, J. A. Hankin, P. M. Henson, and R. C. Murphy. MALDI imaging MS of phospholipids in the mouse lung. J. Lipid Res. 2011. 52: 1551-1560.