DNA microarray analysis of Methanosarcina mazei Go1 reveals adaptation to different methanogenic substrates
DNA microarray analysis of Methanosarcina mazei Go1 reveals adaptation to different methanogenic substrates
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DOI：
10.1007/s00438-005-1126-9
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发表时间：
2005-05-01
影响因子：
3.1
通讯作者：
Deppenmeier, U
中科院分区：
文献类型：
--
作者：
Hovey, R;Lentes, S;Deppenmeier, U
构建了马氏甲烷八叠球菌 Go1 DNA 阵列，并用于评估在两种替代产甲烷底物（乙酸盐或甲醇）中生长的细胞的基因组表达模式，作为唯一的碳和能源。对整个基因组差异转录的分析揭示了两组功能分组的基因，它们与乙酸盐和甲醇代谢的中心生化途径平行，并反映了乙酸盐和甲醇代谢的许多已知特征。这些包括编码乙酸激活酶、乙酰辅酶A合酶/CO脱氢酶和碳酸酐酶的乙酸诱导基因。有趣的是，在乙酸盐上生长期间以显着更高水平表达的其他基因包括两种能量守恒复合物（Ech 氢化酶和 A(1)A(0) 型 ATP 合酶）。许多以前未知的特征包括通过乙酸盐诱导编码铁氧还蛋白和黄素蛋白的基因、醛：铁氧还蛋白氧化还原酶、合成芳香族氨基酸的酶以及铁、钴和寡肽摄取系统的成分。相比之下，甲醇生长的细胞表现出与产甲烷作用的甲基营养途径相关的基因表达升高。相对于乙酸盐，在甲醇培养基中生长的细胞中翻译装置组件的基因表达也有所升高，并且与在前一种基质上观察到的更快的生长速率相关。这些实验首次全面了解产甲烷古菌中底物依赖性基因表达。鉴于目前我们对这些大型古菌基因组的了解还很有限，这种全基因组方法与互补的分子和生化工具相结合，应该会大大加速对甲烷八叠球菌细胞生理学的探索。
Methansarcina mazei Go1 DNA arrays were constructed and used to evaluate the genomic expression patterns of cells grown on either of two alternative methanogenic substrates, acetate or methanol, as sole carbon and energy source. Analysis of differential transcription across the genome revealed two functionally grouped sets of genes that parallel the central biochemical pathways in, and reflect many known features of, acetate and methanol metabolism. These include the acetate-induced genes encoding acetate activating enzymes, acetyl-CoA synthase/CO dehydrogenase, and carbonic anhydrase. Interestingly, additional genes expressed at significantly higher levels during growth on acetate included two energy-conserving complexes (the Ech hydrogenase, and the A(1)A(0)-type ATP synthase). Many previously unknown features included the induction by acetate of genes coding for ferredoxins and flavoproteins, an aldehyde:ferredoxin oxidoreductase, enzymes for the synthesis of aromatic amino acids, and components of iron, cobalt and oligopeptide uptake systems. In contrast, methanol-grown cells exhibited elevated expression of genes assigned to the methylotrophic pathway of methanogenesis. Expression of genes for components of the translation apparatus was also elevated in cells grown in the methanol medium relative to acetate, and was correlated with the faster growth rate observed on the former substrate. These experiments provide the first comprehensive insight into substrate-dependent gene expression in a methanogenic archaeon. This genome-wide approach, coupled with the complementary molecular and biochemical tools, should greatly accelerate the exploration of Methanosarcina cell physiology, given the present modest level of our knowledge of these large archaeal genomes.