Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential
Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential
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DOI：
10.1128/iai.65.7.2707-2716.1997
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发表时间：
1997-07-01
影响因子：
3.1
通讯作者：
Batt, CA
中科院分区：
文献类型：
--
作者：
Wiedmann, M;Bruce, JL;Batt, CA
共133个单核细胞增生李斯特菌菌株的特点是核糖分型和等位基因分析的毒力基因hly，actA，inlA，以揭示独立的系统发育和特定的毒力标记之间的联系。PCR-限制性片段长度多态性分析显示8个hly、11个inlA和2个actA等位基因。这些毒力基因等位基因和核糖体类型的组合将L.单核细胞增多症分为三个不同的谱系。虽然不同的hly和inlA等位基因通常被发现聚类成这三个谱系，actA等位基因分离独立。这三个系统发育谱系被证实时，22个部分actA DNA序列进行了分析。对L.单核细胞增多性菌株显示了三个谱系之间致病潜力差异的证据。谱系I包含李斯特菌病流行期间分离的所有菌株，而谱系III中未发现人类分离株。在所有三个谱系中都发现了动物分离株。我们发现的证据表明，在细胞培养试验中，来自谱系I和In的菌株比谱系II菌株具有更高的噬斑效率。来自谱系III的菌株似乎也比来自谱系II的菌株形成更大的噬斑。一个独特的核糖体型片段和独特的16 S rRNA基因序列进一步表明谱系III可能代表L.单核细胞增生亚种。20个可用的人类分离株中没有一个，但我们的动物分离株中有11%被归为该谱系，表明该谱系中的菌株对人类的毒力可能降低。
A total of 133 Listeria monocytogenes isolates were characterized by ribotyping and allelic analysis of the virulence genes hly, actA, and inlA to uncover linkages between independent phylogenetic and specific virulence markers. PCR-restriction fragment length polymorphisms revealed 8 hly, 11 inlA, and 2 actA alleles. The combination of these virulence gene alleles and ribotype patterns separated L. monocytogenes into three distinct lineages. While distinct hly and inlA alleles were generally found to cluster into these three lineages, actA alleles segregated independently. These three phylogenetic lineages were confirmed when 22 partial actA DNA sequences were analyzed. The clinical history of the L. monocytogenes strains showed evidence for differences in pathogenic potential among the three lineages. Lineage I contains all strains isolated during epidemic outbreaks of listeriosis, while no human isolates were found in lineage III. Animal isolates were found in all three lineages. We found evidence that isolates from lineages I and In have a higher plaquing efficiency than lineage II strains in a cell culture assay. Strains from lineage III also seem to form larger plaques than strains from lineage II. A distinctive ribotype fragment and unique 16S rRNA gene sequences furthermore suggest that lineage III might represent a L. monocytogenes subspecies. None of the 20 human isolates available but 11% of our animal isolates were grouped in this lineage, indicating that strains in this lineage might have reduced virulence for humans.