Anterograde Fast Component of Axonal Transport During Insulin-Induced Hypoglycemia in Nondiabetic and Diabetic Rats
Anterograde Fast Component of Axonal Transport During Insulin-Induced Hypoglycemia in Nondiabetic and Diabetic Rats
复制标题
非糖尿病和糖尿病大鼠胰岛素诱导低血糖期间轴突运输的顺行快速成分
DOI：
10.2337/diab.36.7.853
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发表时间：
1987
期刊：
影响因子：
7.7
通讯作者：
J. Jakobsen
中科院分区：
文献类型：
--
作者：
P. Sidenius;J. Jakobsen
为了阐明与低血糖相关的周围神经病变的发病机制，在急性和长期胰岛素诱导低血糖期间的非糖尿病大鼠以及患有急性低血糖的链佐星糖尿病（STZ-D）大鼠中研究了轴突运输的顺行快速成分（aFC）。将[35S]蛋氨酸和[3H]岩藻糖注射到背根神经节（L5）中，分别标记蛋白质和糖蛋白。在4小时的运输过程中，大腿温度保持恒定。急性严重低血糖 (1.5 ± 0.2 mM) 与 aFC 量减少 36% 相关（测试组为 2.3 ± 0.7%，对照组为 3.6 ± 0.8%），而转运速度不受影响。通过胰岛素预处理 3 天获得的长时间低血糖阻止了 aFC 量的下降。在 STZ-D 大鼠中，急性严重低血糖 (1.5 ± 0.6 mM) 产生类似但不太明显的 aFC 降低。我们的结论是，低血糖与轴突运输的改变有关，轴突运输的改变可能在神经病的发展中发挥作用。长时间的低血糖可以保护轴突运输免受血糖减少的影响，而持续数天未经治疗的糖尿病状态对低血糖发作具有部分保护作用。
To elucidate the pathogenesis of the peripheral neuropathy associated with hypoglycemia the anterograde fast component (aFC) of axonal transport was studied in nondiabetic rats during acute and prolonged insulin-induced hypoglycemia and in streptozocin-diabetic (STZ-D) rats with acute hypoglycemia. [35S]methionine and [3H]fucose were injected into the dorsal root ganglion (L5) to label protein and glycoprotein, respectively. During the 4 h of transport, thigh temperature was maintained constant. Acute severe hypoglycemia (1.5 ± 0.2 mM) was associated with a 36% decrease in the amount of aFC (2.3 ± 0.7% in the test group vs. 3.6 ± 0.8% in the controls), whereas transport velocity was unaffected. Prolonged hypoglycemia, obtained by pretreatment with insulin for 3 days, prevented the decrease in amount of aFC. In STZ-D rats, acute severe hypoglycemia (1.5 ± 0.6 mM) produced a similar but less-pronounced decrease of aFC. We conclude that hypoglycemia is associated with alterations in axonal transport that could play a role in development of neuropathy. Prolonged hypoglycemia protects axonal transport against the effects of glucopenia, and an untreated diabetic state maintained for several days has a partially protective effect against episodes of hypoglycemia.