Stochasticity and homeostasis in the E. coli replication and division cycle.
Stochasticity and homeostasis in the E. coli replication and division cycle.
复制标题
大肠杆菌复制和分裂周期中的随机性和稳态。
DOI：
10.1038/srep18261
复制
发表时间：
2015-12-16
影响因子：
4.6
通讯作者：
Tans SJ
中科院分区：
文献类型：
--
作者：
Adiciptaningrum A;Osella M;Moolman MC;Cosentino Lagomarsino M;Tans SJ
细胞如何纠正随机性，以协调染色体复制和细胞分裂周期知之甚少。我们使用延时显微镜和荧光标记的SeqA来确定出生，起始，终止和分裂的时间，以及整个细胞周期的细胞大小。我们发现，出生和启动（B期）之间的时间补偿出生大小和增长率的随机变异。终止和分裂之间的时间（D期）也弥补了大小和生长的可变性，使复制起始是细胞分裂的主要触发因素的概念无效。相反，启动和终止之间的时间（C期）没有显示这样的补偿。有趣的是，对于自发生长更快的细胞，C期确实显示出小但系统的减少，这表明代谢波动和复制之间存在耦合。采用自回归理论框架比较了不同的子周期控制模型。
How cells correct for stochasticity to coordinate the chromosome replication and cellular division cycle is poorly understood. We used time-lapse microscopy and fluorescently labelled SeqA to determine the timing of birth, initiation, termination, and division, as well as cell size throughout the cell cycle. We found that the time between birth and initiation (B-period) compensates for stochastic variability in birth size and growth rate. The time between termination and division (D-period) also compensates for size and growth variability, invalidating the notion that replication initiation is the principal trigger for cell division. In contrast, the time between initiation and termination (C-period) did not display such compensations. Interestingly, the C-period did show small but systematic decreases for cells that spontaneously grew faster, which suggests a coupling between metabolic fluctuations and replication. An auto-regressive theoretical framework was employed to compare different possible models of sub-period control.