An extreme cytoplasmic bottleneck in the modern European cultivated potato (Solanum tuberosum) is not reflected in decreased levels of nuclear diversity
An extreme cytoplasmic bottleneck in the modern European cultivated potato (Solanum tuberosum) is not reflected in decreased levels of nuclear diversity
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DOI：
10.1098/rspb.1999.0683
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发表时间：
1999-03-22
影响因子：
4.7
通讯作者：
Waugh, R
中科院分区：
文献类型：
--
作者：
Provan, J;Powell, W;Waugh, R
利用叶绿体多态性（CP）和核简单重复序列（SSR）分析了欧洲栽培马铃薯（Solanum tuberosum sap.）tuberosum）。根据烟草叶绿体全序列设计引物，对一系列马铃薯品种进行多态性扩增。结合7个多态性cpSSR位点的数据，得到26个单倍型，其中（单倍型A）占151的178个人研究和对应的T型细胞质先前确定的栽培马铃薯叶绿体限制性片段长度多态性分析。系统发育和多样性分析的cpSSR单倍型之间的关系证实了更高水平的细胞质多样性与T型组。然而，在8个核SSR位点的多样性水平，没有显着不同的细胞质组之间，这表明在现代栽培马铃薯的进化严重的母体瓶颈。这些结果突出了量化细胞质和细胞核多样性水平的重要性，并证实了需要改变育种实践，以增加栽培基因库中非T型细胞质的水平，从而有助于减少与花粉不育相关的问题。这可以通过使用cpSSR的种质分析来促进，这将允许有效地选择不同的细胞质供体。
We have used the polymorphic chloroplast (cp) and nuclear simple sequence repeats (SSRs) to analyse levels of cytoplasmic and nuclear diversity in the gene pool of the European cultivated potato (Solanum tuberosum sap. tuberosum). Primers designed from the complete chloroplast sequence of tobacco (Nicotiana tabacum) were used to amplify polymorphic products in a range of potato cultivars. Combining the data from seven polymorphic cpSSR loci gave 26 haplotypes, one of which (haplotype A) accounted for 151 out of the 178 individuals studied and corresponded to the T-type cytoplasm previously identified in cultivated potatoes using chloroplast restriction fragment length polymorphism analysis. Phylogenetic and diversity analyses of the relationships between cpSSR haplotypes confirmed much higher levels of cytoplasmic diversity outwith the T-type group. Diversity levels at eight nuclear SSR loci, however, were not significantly different between cytoplasmic groups, suggesting a severe maternal bottleneck in the evolution of the modern cultivated potato. These results highlight the importance in quantifying levels of cytoplasmic as well as nuclear diversity and confirm the need for a change in breeding practices to increase levels of non-T-type cytoplasm in the cultivated gene pool, thus helping reduce problems associated with pollen sterility. This may be facilitated by germplasm analysis using cpSSRs, which will allow efficient selection of diverse cytoplasm donors.