Effect of combined alanine and glutamine administration on the inhibition of liver regeneration caused by long-term administration of alcohol.
Effect of combined alanine and glutamine administration on the inhibition of liver regeneration caused by long-term administration of alcohol.
复制标题
丙氨酸和谷氨酰胺联合给药对长期饮酒引起的肝再生抑制的影响。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
K. Mawatari
中科院分区：
文献类型：
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作者：
T. Tanaka;M. Imano;T. Yamashita;T. Monna;S. Nishiguchi;T. Kuroki;S. Otani;K. Maezono;K. Mawatari
我们研究了丙氨酸和谷氨酰胺的混合物的管理对酒精引起的肝再生的抑制进行部分肝切除术后6周开始酒精管理的大鼠的效果。给予酒精的大鼠部分肝切除术后24小时，DNA合成受到抑制，但丙氨酸和谷氨酰胺治疗部分阻止了这种抑制。为了确定这种影响的机制，多胺代谢进行了研究。酒精或丙氨酸加谷氨酰胺的管理没有影响的活性，鸟氨酸脱羧酶，多胺代谢的限速酶。在肝脏中，三种多胺中，只有精胺浓度变化显著。在长期服用酒精的过程中，它会下降，而这种下降可以通过丙氨酸和谷氨酰胺治疗来防止。乙醇会增加亚精胺的乙酰化产物N（1）-乙酰亚精胺的水平，而给予丙氨酸和谷氨酰胺时，其升高幅度明显较小。肝脏精脒/精胺N（1）-乙酰转移酶是多胺乙酰化的关键酶，酒精可诱导肝脏精脒/精胺N（1）-乙酰转移酶的活性，丙氨酸和谷氨酰胺可抑制酒精对肝脏精脒/精胺N（1）-乙酰转移酶的诱导作用。结果表明，丙氨酸和谷氨酰胺处理可以帮助防止酒精引起的肝再生抑制，通过维持精胺水平和抑制亚精胺的乙酰化。
We studied the effect of administration of a mixture of alanine and glutamine on the inhibition of liver regeneration caused by alcohol in rats undergoing partial hepatectomy 6 weeks after the start of alcohol administration. DNA synthesis was inhibited 24 hr after partial hepatectomy in rats given alcohol, but treatment with alanine and glutamine partially prevented this inhibition. To identify the mechanism of this effect, polyamine metabolism was studied. Administration of alcohol or alanine plus glutamine had no effect on the activity of ornithine decarboxylase, a rate-limiting enzyme of polyamine metabolism. In the liver, of the three polyamines, only the spermine concentration changed significantly. It decreased during long-term administration of alcohol, and this decrease was prevented by treatment with alanine and glutamine. The level of N(1)-acetylspermidine, the acetylated product of spermidine, was increased by alcohol, and its elevation was significantly less when alanine and glutamine were given. Hepatic spermidine/spermine N(1)-acetyltransferase, the key enzyme of polyamine acetylation, was induced by long-term administration of alcohol, and this induction was suppressed by alanine plus glutamine. The results suggest that treatment with alanine and glutamine can help to prevent the inhibition of liver regeneration caused by alcohol by maintaining the spermine level and suppressing the acetylation of spermidine.