Protective effect of sevoflurane on hepatic ischaemia/reperfusion injury in the rat: A dose–response study
Protective effect of sevoflurane on hepatic ischaemia/reperfusion injury in the rat: A dose–response study
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七氟醚对大鼠肝缺血/再灌注损伤的保护作用：剂量反应研究
DOI：
10.1097/eja.0b013e3283614023
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发表时间：
2013
影响因子：
3.6
通讯作者：
Hua Liu
中科院分区：
文献类型：
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作者：
Shao;P. Jiang;Lu Liu;Hua Liu
背景：缺血/再灌注损伤是肝脏手术中的常见问题。了解七氟醚剂量在预处理和随后对缺血/再灌注损伤的肝保护中的作用将是有用的。目的探讨不同剂量七氟醚预处理对大鼠肝脏缺血再灌注损伤的保护作用。设计随机、对照、实验室研究。中山大学附属第五医院麻醉科，广东省珠海市。实验对象50只体重200 ~ 250克的雄性sd大鼠，随机分为五组。对照组（假手术，无缺血/再灌注）、I/R组（缺血/再灌注但无七氟醚预处理）、S1[1最小肺泡浓度(MAC) = 2.4%]、S2 （1.5 MAC = 3.6%）和S3 （2 MAC = 4.8%）组分别进行七氟醚预处理，缺血60 min，再灌注120 min。再灌注结束时，测定血清丙氨酸转氨酶和天冬氨酸转氨酶水平，以及肝脏超氧化物歧化酶活性、髓过氧化物酶和丙二醛含量。同时进行肝脏组织学检查。结果与I/R组相比，七氟醚组血清天冬氨酸转氨酶和丙氨酸转氨酶水平显著降低（P < 0.05）。在肝脏中，所有七氟醚浓度均显著降低了I/ r诱导的髓过氧化物酶活性和丙二醛水平的升高（P < 0.05）。七氟醚预处理均能抑制I/R诱导的超氧化物歧化酶活性降低（P < 0.05）。S1、S2和S3组在上述变量中均无显著差异。结论七氟醚预处理对肝缺血再灌注损伤有保护作用，但在浓度范围内无显著的剂量-效应关系。在较低浓度下可能存在剂量-反应关系。
BACKGROUND Ischaemia/reperfusion injury is a common problem in hepatic surgery. An appreciation of the role of sevoflurane dose in preconditioning and subsequent hepatoprotection against ischaemia/reperfusion injury would be useful. OBJECTIVE The aim of current study was to investigate the protective effect of sevoflurane preconditioning at different doses on hepatic ischaemia/reperfusion injury in rats. DESIGN Randomised, controlled, laboratory study. SETTING The Department of Anaesthesiology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, China. PARTICIPANTS Fifty male Sprague–Dawley rats weighing 200 to 250 g, randomly assigned to five groups. INTERVENTIONS Control group (sham surgery, no ischaemia/reperfusion), I/R group (ischaemia/reperfusion but no sevoflurane pretreatment), S1 [1 minimum alveolar concentration (MAC) = 2.4%], S2 (1.5 MAC = 3.6%) and S3 (2 MAC = 4.8%) groups with sevoflurane pretreatment, respectively, followed by 60 min ischaemia and 120 min reperfusion. MAIN OUTCOME MEASURES At the end of reperfusion, serum levels of alanine aminotransferase and aspartate aminotransferase as well as superoxide dismutase activity, myeloperoxidase and malondialdehyde content in the liver were determined. Histological examination of the liver was also performed. RESULTS Serum levels of aspartate aminotransferase and alanine aminotransferase in the sevoflurane groups were significantly reduced compared to the elevated levels seen in the I/R group (P < 0.05). In the liver, the I/R-induced increase in myeloperoxidase activity and malondialdehyde level were significantly reduced by all sevoflurane concentrations (P < 0.05). The decrease in superoxide dismutase activity induced by I/R was prevented by all sevoflurane pretreatments (P < 0.05). No significant differences between the S1, S2 and S3 groups were seen in any of the above variables. CONCLUSION Sevoflurane pretreatment exerts a protective effect on hepatic ischaemia/reperfusion injury but there is no significant dose–response relationship in the concentration range used. It is possible that a dose–response relationship might exist at lower concentrations.
缺血预处理可增强长期缺血损伤大鼠肝脏中肝细胞的再生能力。
DOI：
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发表时间：
2006
期刊：
Journal of Gastroenterology and Hepatology (In press)
影响因子：
--
作者：
Yamada F;Saito T;Abe T;et al.
通讯作者：
et al.