The mechanism of polyplex internalization into cells: testing the GM1/caveolin-1 lipid raft mediated endocytosis pathway.
The mechanism of polyplex internalization into cells: testing the GM1/caveolin-1 lipid raft mediated endocytosis pathway.
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DOI：
10.1021/mp900241t
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发表时间：
2010-02-01
影响因子：
4.9
通讯作者：
Holl MM
中科院分区：
文献类型：
--
作者：
Qi R;Mullen DG;Baker JR;Holl MM
利用Cos-7、293A、C6、HeLa、KB和HepG2细胞系，探讨了GM1/caveolin-1脂筏介导的第5代和第7代聚（氨基胺）树突状多聚物的内吞机制。采用Dot Blot和Western Blot分别检测GM1和caveolin-1的表达水平。用外源性GM1或神经节苷脂抑制剂PPMP孵育调节细胞膜GM1水平，用腺病毒载体表达的cavo -1（AdCav-1）上调CAV-1水平。所有病例均以霍乱毒素B亚基为阳性对照。没有证据表明GM1/caveolin-1脂质筏介导了第5代和第7代聚（氨基胺）树状聚合物多聚体的内吞机制。
The GM1/caveolin-1 lipid raft mediated endocytosis mechanism was explored for generation 5 and 7 poly(amidoamine) dendrimer polyplexes employing the Cos-7, 293A, C6, HeLa, KB, and HepG2 cell lines. Expression levels of GM1 and caveolin-1 were measured using Dot Blot and Western Blot, respectively. The level of GM1 in the cell plasma membrane was adjusted by incubation with exogenous GM1 or ganglioside inhibitor PPMP and the level of CAV-1 was adjusted by up-regulation with the adenovirus vector expressed Caveolin-1(AdCav-1). Cholera toxin B subunit was employed as a positive control for uptake in all cases. No evidence was found for a GM1/caveolin-1 lipid raft mediated endocytosis mechanism for the generation 5 and 7 poly(amidoamine) dendrimer polyplexes.
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