Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry
Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry
复制标题
呼气醛的质谱学分析用护理试纸
DOI：
10.1021/acs.analchem.1c01011
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发表时间：
2021-06-24
影响因子：
7.4
通讯作者：
Nie, Zongxiu
中科院分区：
文献类型：
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作者：
Li, Zhengzhou;Li, Yuze;Nie, Zongxiu
呼出气（EB）中的挥发性有机化合物（VOC）被认为是肺部疾病有前途的生物标志物。一种方便、灵敏的 EB VOC 即时护理 (POC) 测试方法至关重要。在这里，我们开发了一种用于分析 EB 醛的 POC 试纸，EB 醛是肺癌的潜在生物标志物。将探针分子 4-氨基苯硫酚 (4-ATP) 锚定在纸质基底上，通过席夫碱反应特异性捕获气相醛。同时，利用薄膜反应加速来提高捕获效率。通过喷纸将试纸直接耦合至质谱仪，获得了高灵敏度（0.1 ppt）和宽量化线性范围（从10 ppt至1 ppm）。使用该试纸对肺癌患者的 EB 进行分析显示，与健康志愿者的 EB 相比，几种报告的醛标记物显着增加，表明该方法具有灵敏、低成本且方便的肺癌筛查和诊断的潜力。
Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. A probe molecule, 4-aminothiophenol (4-ATP), was anchored on a paper substrate to specifically capture gas-phase aldehydes through the Schiff base reaction. Meanwhile, thin-film reaction acceleration was utilized to increase capture efficiency. By directly coupling the test paper to a mass spectrometer through paper spray, high sensitivity (0.1 ppt) and a wide quantification linear range (from 10 ppt to 1 ppm) were obtained. Analysis of EB from lung cancer patients with the test paper showed a significant increase in several reported aldehyde markers compared to EB from healthy volunteers, indicating the potential of this method for sensitive, low-cost, and convenient lung cancer screening and diagnosis.