Maternally transmitted non-bacterial male killer in Drosophila biauraria
Maternally transmitted non-bacterial male killer in Drosophila biauraria
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DOI：
10.1098/rsbl.2017.0476
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发表时间：
2017-10-01
期刊：
影响因子：
3.3
通讯作者：
Watada, Masayoshi
中科院分区：
文献类型：
--
作者：
Kageyama, Daisuke;Yoshimura, Kanamu;Watada, Masayoshi
一种母系遗传的全雌性特征在节肢动物中广泛存在，这是由细菌内共生体如沃尔巴克氏体、立克次体、螺原体和Cardinium引起的。我们发现了一只采自日本北海道Tomakomai的雌性双尾果蝇，它产生了全雌性的后代。这一全雌性性状通过与来自同一群体的正常系（SP11-20）的雄性以1：1的性比回交而在等雌性系（SP12 F）中母系遗传。连续两代四环素处理对全雌性状没有影响。然而，将SP12 F雌性动物的过滤灭菌匀浆显微注射到SP11-20雌性动物中建立了全雌性母系。我们的数据表明，传播媒介的作用，最有可能是病毒，但不是细菌或原生生物，作为全女性表型的可能原因，这可能是通过杀死男性胚胎，因为SP12 F的卵孵化率几乎是SP11-20的一半。这是双翅目中首次证实母系遗传的病毒样元件是导致D.双尾虫
A maternally inherited, all-female trait is widely found among arthropods, which is caused by bacterial endosymbionts such as Wolbachia, Rickettsia, Spiroplasma and Cardinium. We discovered a single female of Drosophila biauraria, collected from Tomakomai, Hokkaido, Japan, that produced all-female offspring. This all-female trait was maternally inherited in the iso-female line (SP12F) by backcrossing with males of a normal line (SP11-20) with a 1 : 1 sex ratio derived from the same population. The all-female trait was not affected by tetracycline treatment performed for two consecutive generations. However, the microinjection of filter-sterilized homogenate of SP12F females into SP11-20 females established all-female matrilines. Our data suggest the role of transmissible agents, most likely viruses, but not bacteria or protists, as the possible cause of the all-female phenotype, which is likely to be achieved by killing of male embryos because egg hatch rates of SP12F were nearly half those of SP11-20. This is the first report in Diptera to demonstrate a maternally inherited virus-like element as the cause of the male-killing phenotype in D. biauraria.