Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism.
Pure tocotrienol concentrate protected rat gastric mucosa from acute stress-induced injury by a non-antioxidant mechanism.
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DOI：
10.5114/pjp.2013.34604
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发表时间：
2013-04
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通讯作者：
Mohd Nor Syidiq Rodzian;Ibrahim Abdel Aziz Ibrahim-Ibrahim-Abdel-Aziz-Ibrahim-7618389;Mohd Fahami Nur Azlina;M. Nafeeza
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文献类型：
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作者：
Mohd Nor Syidiq Rodzian;Ibrahim Abdel Aziz Ibrahim-Ibrahim-Abdel-Aziz-Ibrahim-7618389;Mohd Fahami Nur Azlina;M. Nafeeza
压力已被认为是各种主要健康问题的危险因素，如压力引起的胃粘膜损伤。本研究观察了由90% δ-生育三烯酚和10% γ-生育三烯酚组成的生育三烯酚（T3）浓缩纯品对水浸束缚应激（WIRS）所致大鼠胃损伤的作用。将14只雄性Sprague-Dawley大鼠（200-250 g）分为两个相等的组：对照组和治疗组。治疗组每天接受60 mg/kg体重的T3浓缩物，持续28天。在给药前每天记录大鼠的体重。在给药期结束时，所有大鼠均接受WIRS 3.5小时，随后对大鼠实施安乐死。分离胃，沿胃大弯沿着切开，检查胃粘膜病变，并测定胃粘膜丙二醛（MDA）和前列腺素E β（PGE β）含量。治疗组大鼠胃粘膜损伤指数明显低于对照组。这表明T3浓缩物具有保护胃粘膜免受急性应激诱导的胃损伤的能力。治疗前后体重变化无显著差异。两组胃内PGE 2含量相当。然而，与对照组相比，处理组的胃MDA含量显著更高，表明T3补充不能减少脂质过氧化过程。本研究得出结论，T3浓缩物具有通过非抗氧化机制保护胃粘膜免受应激诱导的损伤的能力。
Stress has been implicated as a risk factor of various major health problems, such as stress-induced gastric mucosal injury. This study was performed to investigate the action of a pure preparation of tocotrienol (T3) concentrate, made up of 90% δ-tocotrienol and 10% γ-tocotrienol, on gastric injury of rats induced by water-immersion restraint stress (WIRS). Fourteen male Sprague-Dawley rats (200-250 g) were divided into two equal groups: a control group and a treated group. The treatment group received T3 concentrate at 60 mg/kg body weight daily for 28 days. The body weights of rats were recorded daily before the treatment was given. At the end of the treatment period, all rats were subjected to WIRS for 3.5 hours, following which the rats were euthanized. The stomachs were isolated and opened along the greater curvature for the examination of lesions and measurements of gastric malondialdehyde (MDA) and prostaglandin E₂ (PGE₂) contents. The mean gastric mucosal lesion index in the treated rats was significantly lower than that in the control rats. This suggests that the T3 concentrate has the ability to confer protection to the gastric mucosa against gastric injury induced by acute stress. No significant difference was observed for changes in body weight before and after the treatment. The gastric PGE2 content in both groups was comparable. However, the gastric MDA content was significantly higher in the treated group compared to the control group, indicating that the T3 supplementation was not able to reduce the lipid peroxidation process. This study concludes that the T3 concentrate has the ability to protect the gastric mucosa from stress-induced injury by a non-antioxidant mechanism.