The vrille gene of Drosophila is a maternal enhancer of decapentaplegic and encodes a new member of the bZIP family of transcription factors.
The vrille gene of Drosophila is a maternal enhancer of decapentaplegic and encodes a new member of the bZIP family of transcription factors.
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作者：
H. George;R. Terracol
我们在这里报告了一个新的果蝇合子致死位点 vrille (vri) 的遗传和分子特征。 Vri 等位基因不仅作为由复活节和十肢瘫痪 (dpp) 突变引起的胚胎背腹模式缺陷的显性母体增强子，而且作为翅膀表型的 dpp 等位基因的显性合子增强子。 vri 基因编码 bZIP 转录因子家族的新成员，与非洲爪蟾基因 9 密切相关，在蝌蚪尾部吸收程序期间由甲状腺激素诱导，以及 NF-IL3A（一种反式激活白细胞介素 3 启动子的人类 T 细胞转录因子）。 NF-IL3A 与 Vri 的一段 68 个氨基酸（包括 bZIP 结构域）具有 93% 的相似性和 60% 的同一性。尽管所有测试的等位基因都表现得像反形体，但显性增强也出现在阻止 bZIP 结构域翻译的无义突变等位基因中。由于胚胎和翅膀中vri等位基因对dpp表型的强烈结构域增强，以及vri和短静脉dpp等位基因引起的翅膀静脉表型之间的相似性，我们假设vri直接或间接与dpp（TGFβ同源物）信号转导途径的某些成分相互作用。
We report here the genetical and molecular characterization of a new Drosophila zygotic lethal locus, vrille (vri). Vri alleles act not only as dominant maternal enhancers of embryonic dorsoventral patterning defects caused by easter and decapentaplegic (dpp) mutations, but also as dominant zygotic enhancers of dpp alleles for phenotypes in wing. The vri gene encodes a new member of the bZIP family of transcription factors closely related to gene 9 of Xenopus laevis, induced by thyroid hormone during the tadpole tail resorption program, and NF-IL3A, a human T cell transcription factor that transactivates the interleukin-3 promoter. NF-IL3A shares 93% similarity and 60% identity with Vri for a stretch of 68 amino acids that includes the bZIP domain. Although all the alleles tested behave like antimorphs, the dominant enhancement is also seen with a nonsense mutation allele that prevents translation of the bZIP domain. Because of the strong domainant enhancement of dpp phenotypes by vri alleles in both embryo and wing, and also the similarity between the wing vein phenotypes caused by the vri and shortvein dpp alleles, we postulate that vri interacts either directly or indirectly with certain components of the dpp (a TGF beta homologue) signal transduction pathway.