Expression of type XII collagen and hemidesmosome-associated proteins in keratoconus corneas
Expression of type XII collagen and hemidesmosome-associated proteins in keratoconus corneas
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DOI：
10.1076/ceyr.22.5.333.5491
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发表时间：
2001-05-01
影响因子：
2
通讯作者：
Yue, BYJT
中科院分区：
文献类型：
--
作者：
Cheng, EL;Maruyama, I;Yue, BYJT
目的。圆锥角膜。是一种以中央和旁中央角膜变薄以及晚期病例形成疤痕为特征的疾病。本研究旨在检测圆锥角膜中 XII 型胶原蛋白、半桥粒相关蛋白和 β1 整合素的表达。方法。从正常受试者和患有圆锥角膜和其他角膜疾病的患者中收集角膜纽扣。对冰冻切片进行 XII 型胶原、大疱性类天疱疮抗原 (BP180) 和整合素亚基 α6、β4 和 β1 的免疫荧光染色。 结果。所有检测到的蛋白质都不同程度地在正常人角膜中表达。圆锥角膜上皮基底膜区和基质基质中 XII 型胶原蛋白的染色强度减弱。 BP180 或整联蛋白 alpha6、beta4 和 beta1 的染色模式或强度均未发现显着变化。结论。圆锥角膜的上皮基底膜区和基质基质中 XII 型胶原蛋白的水平降低。这些改变可能会影响角膜上皮与底层基底膜的关键相互作用，以及基质中的细胞-基质相互作用和基质组织。
Purpose. Keratoconus. is a disease characterized by thinning of the central and paracentral cornea and scarring in advanced cases. This study was performed to examine the expression of type XII collagen, proteins associated with hemidesmosomes, and beta1 integrin in keratoconus corneas.Methods. Corneal buttons were collected from normal subjects and patients with keratoconus and other corneal diseases. Immunofluorescence staining was performed on frozen sections for type XII collagen, bullous pemphigoid antigen (BP180), and integrin subunits alpha6, beta4, and beta1.Results. To varying degrees, all proteins examined were expressed in normal human corneas. The staining intensity of type XII collagen was diminished in keratoconus corneas in the epithelial basement membrane zone and the stromal matrix. No significant variation was found in either the staining patterns or intensities for BP180, or integrins alpha6, beta4, and beta1.Conclusions. The level of type XII collagen was reduced in the epithelial basement membrane zone and stromal matrices in keratoconus corneas. These alterations may affect critical interactions of the corneal epithelium with the underlying basement membrane, and cell-matrix interactions and matrix organization in the stroma.