Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH
Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH
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DOI：
10.1007/978-1-61779-513-8_13
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Hansson, Gunnar C.
中科院分区：
文献类型：
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作者：
Johansson, Malin E. V.;Hansson, Gunnar C.
由于粘液是高度水合的，因此在免疫染色期间必须特别注意在组织学制备中保存粘液。在这里，我们描述了如何固定组织的方式，粘液是保存在石蜡包埋的组织。我们还描述了如何在粘液中的主要大分子成分，粘蛋白，免疫染色，以及如何细菌可以定位在与保存粘液的制剂，通过荧光原位杂交。
As mucus is highly hydrated, special care has to be taken to preserve this in histological preparations during immunostaining. Here, we describe how to fix tissues in such a way that the mucus is preserved in paraffin-embedded tissue. We also describe how the major macromolecular components in the mucus, the mucins, are immunostained and how bacteria can be localized in preparations with preserved mucus by fluorescent in situ hybridization.