Thyroglobulin antibodies in serum of patients with differentiated thyroid cancer: Relationship between epitope specificities and thyroglobulin recovery
Thyroglobulin antibodies in serum of patients with differentiated thyroid cancer: Relationship between epitope specificities and thyroglobulin recovery
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DOI：
10.1373/clinchem.2004.044511
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发表时间：
2005-04-01
影响因子：
9.3
通讯作者：
Lazarus, JH
中科院分区：
文献类型：
--
作者：
Okosieme, OE;Evans, C;Lazarus, JH
背景：血清抗甲状腺球蛋白抗体（TgAbs）在分化型甲状腺癌（DTC）患者中很常见，并可干扰甲状腺球蛋白（Tg）测定。我们从DTC患者的tgab阳性血清中鉴定了Tg上的表位，并研究了表位特异性模式与Tg恢复之间的关系。方法：我们检测了50份DTC血清的Tg表位特异性、Tg抗体和Tg回收率。表位识别是通过使用一组10个特性良好的Tg单克隆抗体，在与测试血清的竞争反应中针对6个Tg抗原簇（I-VI）来确定的。Tg采用甲状腺球蛋白IRMA （CIS bio international）测定。添加Tg （TgREC）的回收率由内部测定法确定。结果：58%的患者表位识别仅限于免疫优势簇，而其余患者要么是广泛异质性的（16%），要么是非反应性的（26%）。限制特异性和无限制特异性血清的中位Tg恢复无差异（69% vs 80%; P < 0.05）。TgREC与血清识别的表位总数呈负相关（r = -0.66; P < 0.001）。结论：在DTC患者中存在限制性和广泛特异性的tgb。TgAb干扰与血清识别的表位数量有关，而与表位识别的模式无关。(c) 2005年美国临床化学协会。
Background: Serum antibodies against thyroglobulin (TgAbs) are common in patients with differentiated thyroid cancer (DTC) and can interfere in thyroglobulin (Tg) assays. We identified the epitopes on Tg recognized by TgAb-positive sera from patients with DTC and examined the association between epitope specificity patterns and Tg recovery.Methods: We tested 50 DTC sera for Tg epitope specificity, TgAbs, and Tg recovery. Epitope recognition was determined by use of a panel of 10 well-characterized Tg monoclonal antibodies directed against 6 Tg antigenic clusters (I-VI) in competitive reactions with test sera. Tg was measured by the Thyroglobuline IRMA (CIS bio international). Recovery of added Tg (TgREC) was determined by an in-house assay.Results: Epitope recognition was restricted to immunodominant clusters in 58% of patients, whereas the rest were either broadly heterogeneous (16%) or nonreactive (26%). Median Tg recovery did not differ between sera with restricted and unrestricted specificities (69% vs 80%; P > 0.05). TgREC was inversely correlated with the total number of epitopes recognized by sera (r = -0.66; P < 0.001).Conclusions: TgAbs with both restricted and broad specificities are present in patients with DTC. TgAb interference is related to the number of epitopes recognized by sera rather than the pattern of epitope recognition. (c) 2005 American Association for Clinical Chemistry.