LETHAL PHOTOSENSITIZATION OF BACTERIA IN SUBGINGIVAL PLAQUE FROM PATIENTS WITH CHRONIC PERIODONTITIS
LETHAL PHOTOSENSITIZATION OF BACTERIA IN SUBGINGIVAL PLAQUE FROM PATIENTS WITH CHRONIC PERIODONTITIS
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DOI：
10.1111/j.1600-0765.1993.tb01070.x
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发表时间：
1993-05-01
影响因子：
3.5
通讯作者：
WILSON, M
中科院分区：
文献类型：
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作者：
SARKAR, S;WILSON, M
慢性牙周炎患者的龈下菌斑标本用7.3 mW氦/霓虹激光照射30 S，同时加入50µg/ml甲苯胺蓝O作为光敏剂。照射前后分别对不同菌群和菌种进行活菌计数。经辐照的30个等量的初始活菌数中位数分别为1.13×10(5)CFU(需氧菌)、4.08×10(5)CFU(厌氧菌)、4.92×10(3)CFU(黑色素厌氧菌)、4.75×10(2)CFU(牙龈卟啉单胞菌)、6.15×10(3)CFU(核梭杆菌)和1.7×10(4)CFU(链球菌)。染料/激光的组合显著降低了这些微生物的生存能力，其中需氧菌活菌数的中位数减少了91.1%，厌氧菌减少了96.6%，黑色素厌氧菌减少了100%，牙龈假单胞菌和核假单胞菌减少了94.2%，链球菌减少了94.2%。总体而言，仅靠染料并没有显著降低20个斑块样本中细菌的活力。然而，在一小部分样本中，有迹象表明不受光影响的、染料诱导的毒性。低功率激光联合适当的光敏剂，如果在活体内能够达到对抗牙周下菌斑细菌的类似效果，那么在治疗炎性牙周病时，低功率激光可能是机械清创的有用辅助工具。
Subgingival plaque samples from patients with chronic periodontitis were exposed to light from a 7.3 mW Helium/Neon laser for 30 s in the presence and absence of 50 mug/ml toluidine blue O as a photosensitizer. Viable counts of various groups and species of bacteria were carried out before and after irradiation. The median numbers of viable bacteria initially present in the 30-mul aliquots irradiated were 1.13 x 10(5) cfu (aerobes), 4.08 x 10(5) cfu (anaerobes), 4.92 x 10(3) cfu (black-pigmented anaerobes), 4.75 x 10(2) cfu (Porphyromonas gingivalis), 6.15 x 10(3) cfu (Fusobacterium nucleatum) and 1.7 x 10(4) cfu (streptococci). The dye/laser combination achieved significant reductions in the viability of these organisms, the median reductions in the viable counts being 91.1% for aerobes, 96.6% for anaerobes, 100% for black-pigmented anaerobes, P gingivalis and F. nucleatum and 94.2% for streptococci. Overall, the viability of bacteria in the 20 plaque samples was not significantly decreased by the dye alone. However, in a small minority of samples there were indications of light-independent, dye-induced toxicity. Low-power lasers, in conjunction with appropriate photosensitizers, may be a useful adjunct to mechanical debridement in the treatment of inflammatory periodontal diseases if a similar effectiveness against subgingival plaque bacteria can be achieved in vivo.