Biological and chemical standardization of a hop (Humulus lupulus) botanical dietary supplement
Biological and chemical standardization of a hop (Humulus lupulus) botanical dietary supplement
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DOI：
10.1002/bmc.3177
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发表时间：
2014-06-01
影响因子：
1.8
通讯作者：
van Breemen, Richard B.
中科院分区：
文献类型：
--
作者：
Krause, Elizabeth;Yuan, Yang;van Breemen, Richard B.
出于对传统雌激素替代疗法的安全性的担忧，女性正在使用植物膳食补充剂作为治疗潮热等更年期症状的替代方案。在对植物膳食补充剂的安全性和功效进行临床评估之前，需要经过植物学认证和标准化形式。为了满足对标准化雌激素植物膳食补充剂的需求，开发了啤酒花提取物（Humulus lupulus L.）。尽管啤酒花提取物在啤酒酿造中很有价值，但它也可用作抗焦虑药和安眠药，并且含有公认的雌激素成分。从酿造业中使用的啤酒花品种开始，废啤酒花（提取苦酸后剩余的残留物）被配制成植物性膳食补充剂，然后进行化学和生物标准化。生物标准化利用石川细胞系中雌激素依赖性碱性磷酸酶的诱导。化学标准化基于啤酒花中的异戊烯化酚，其中包括雌激素8-异戊二烯柚皮素、其异构体6-异戊二烯柚皮素、前雌激素异黄腐酚及其异构查尔酮黄腐酚，所有这些均使用高效液相色谱-串联质谱法进行测量。该过程的产品是一种可重复的植物提取物，适合后续的安全性和有效性研究。版权所有 (c) 2014 约翰·威利父子有限公司
Concerned about the safety of conventional estrogen replacement therapy, women are using botanical dietary supplements as alternatives for the management of menopausal symptoms such as hot flashes. Before botanical dietary supplements can be evaluated clinically for safety and efficacy, botanically authenticated and standardized forms are required. To address the demand for a standardized, estrogenic botanical dietary supplement, an extract of hops (Humulus lupulus L.) was developed. Although valued in the brewing of beer, hop extracts are used as anxiolytics and hypnotics and have well-established estrogenic constituents. Starting with a hop cultivar used in the brewing industry, spent hops (the residue remaining after extraction of bitter acids) were formulated into a botanical dietary supplement that was then chemically and biologically standardized. Biological standardization utilized the estrogen-dependent induction of alkaline phosphatase in the Ishikawa cell line. Chemical standardization was based on the prenylated phenols in hops that included estrogenic 8-prenylnaringenin, its isomer 6-prenylnaringenin, and pro-estrogenic isoxanthohumol and its isomeric chalcone xanthohumol, all of which were measured using high-performance liquid chromatography-tandem mass spectrometry. The product of this process was a reproducible botanical extract suitable for subsequent investigations of safety and efficacy. Copyright (c) 2014 John Wiley & Sons, Ltd.