Biological activity of rhBMP-2 released from PLGA microspheres
Biological activity of rhBMP-2 released from PLGA microspheres
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DOI：
10.1115/1.429662
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发表时间：
2000-06-01
影响因子：
1.7
通讯作者：
Yaszemski, MJ
中科院分区：
文献类型：
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作者：
Oldham, JB;Lu, L;Yaszemski, MJ
人重组骨形态发生蛋白-2(rhBMP-2)已被证实能有效地刺激骨骼和骨外部位的骨再生。通过可生物降解的聚(DL-乳酸-乙醇酸)(PLGA)微球的包裹和释放，rhBMP-2的局部浓度可以保持在最佳水平，以刺激不同临床环境愈合部位的骨再生和重塑。PLGA是一种公认的持续输送各种蛋白质的载体。因此，本工作的目的是研究重组人骨形态发生蛋白-2在PLGA微球中的包封率及其释放后的生物学活性。在模拟体液中进行体外实验，发现PLGA微球释放的rhBMP-2对成骨细胞培养的影响与模拟体液中由未包裹的含水的rhBMP-2组成的阳性对照所产生的作用在统计学上相似。这阐明了骨骼组织工程策略中的重要一步，目的是使用包裹的重组人骨形态发生蛋白-2来刺激骨再生和重建。
Human recombinant bone morphogenetic protein-2 (rhBMP-2) has been proven effective ill stimulating the regeneration of bone in both skeletal and extraskeletal locations. Through encapsulation within, and release from, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) microspheres, a proven vehicle for sustained delivery of various proteins, the local concentrations of rhBMP-2 could be maintained at optimal levels to stimulate bone regeneration and remodeling at the site of healing irt diverse clinical settings. Thus the purpose of this work was to investigate the encapsulation of rhBMP-2 in PLGA microspheres and its biologic activity upon release. Using in vitro tests in simulated body fluids, the effect of rhBMP-2 released from PLGA microspheres upon osteoblast cell cultures was found to be statistically similar to the effect produced by positive controls consisting of nonencapsulated aqueous rhBMP-2 in simulated body fluids. This clarifies an important step in skeletal tissue engineering strategies aimed at the use of encapsulated rhBMP-2 to stimulate bone regeneration and remodeling.