Olfactory ensheathing cells promote collateral axonal branching in the injured adult rat spinal cord
Olfactory ensheathing cells promote collateral axonal branching in the injured adult rat spinal cord
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DOI：
10.1016/j.expneurol.2003.09.008
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发表时间：
2004-01-01
影响因子：
5.3
通讯作者：
West, AK
中科院分区：
文献类型：
--
作者：
Chuah, MI;Choi-Lundberg, D;West, AK
近年来，将嗅鞘细胞（EC）注射到脊髓已被用作促进受损轴突再生的实验策略。在这项研究中，我们比较了移植封装内皮细胞和直接注射到脊髓中的效果。在 T8-9 处切开成年大鼠的背柱，实验组的大鼠接受充满 EC 的多孔聚合物胶囊或充满培养基 (CM) 的胶囊，并在损伤部位注射 EC。对照大鼠分为三组：(1)未受伤，(2)移植有CM填充胶囊的损伤组，(3)移植有CM填充胶囊并注射CM的损伤组。受伤三周后，将 Fluororuby 注射到后肢运动和体感皮层以标记皮质脊髓神经元。观察结果表明，EC 处理组中有一些再生纤维，最多可达 10 条。在接受封装内皮细胞的大鼠中，再生纤维与胶囊密切相关。与相应的对照、CM注射组相比，接受 EC 注射的大鼠表现出来自完整腹侧皮质脊髓束 (vCST) 的侧支分支数量显着增加 (P = 0.003)，而在接受封装 EC 的大鼠中观察到侧支分支增加的趋势 (P = 0.07)。 (C) 2003 Elsevier Inc. 保留所有权利。
In recent years, injection of olfactory ensheathing cells (ECs) into the spinal cord has been used as an experimental strategy to promote regeneration of injured axons. In this study, we have compared the effects of transplanting encapsulated ECs with those injected directly into the spinal cord. The dorsal columns of adult rats were cut at T8-9 and rats in experimental groups received either EC-filled porous polymer capsules or culture medium (CM)-filled capsules with ECs injected at the injury site. Control rats were in three groups: (1) uninjured, (2) lesion with transplantation of CM-filled capsules and (3) lesion with transplantation of CM-filled capsules and injections of CM. Three weeks after injury, Fluororuby was injected into the hindlimb motor and somatosensory cortex to label corticospinal neurons. Observations indicated that there were a few regenerating fibres, up to 10, in the EC-treated groups. In rats that received encapsulated ECs, regenerating fibres were present in close association with the capsule. Rats that received EC injections demonstrated a significant increase in the number of collateral branches from the intact ventral corticospinal tract (vCST) compared with the corresponding control, CM-injected group (P = 0.003), while a trend for increased collateral branches was observed in rats that received encapsulated ECs (P = 0.07). (C) 2003 Elsevier Inc. All rights reserved.