Effects of restrictions on maternal feed intake on the immune indexes of umbilical cord blood and liver Toll-like receptor signaling pathways in fetal goats during pregnancy
Effects of restrictions on maternal feed intake on the immune indexes of umbilical cord blood and liver Toll-like receptor signaling pathways in fetal goats during pregnancy
复制标题
母羊孕期限制采食量对胎羊脐血免疫指标及肝脏Toll样受体信号通路的影响
DOI：
10.1186/s40104-019-0336-7
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发表时间：
2019-04-16
影响因子：
7
通讯作者：
Tan, Zhiliang
中科院分区：
文献类型：
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作者：
Chen, Wenxun;Yan, Qiongxian;Tan, Zhiliang
背景肝脏在胎儿发育和出生后具有重要的免疫功能。然而，关于母体营养不良对胎肝免疫功能影响的报道很少。本试验选用12只妊娠山羊(湘东黑山羊，妊娠45d)，随机分为对照组(100%饲喂营养需要量)和限制组(60%饲喂对照组)，试验期55~100d。妊娠100d取胎羊，测定脐血和肝脏Toll样受体(TLR)信号通路的免疫指标和氨基酸组成。结果禁食组母羊体重低于对照组(P<； )。母体饲料摄入量限制降低了脐带血的心脏重量、心脏指数、碱性磷酸酶和血清淀粉样蛋白A(P <；005)。限制组仅组氨酸降低(P= 0.084.0 5)，其他氨基酸含量两组间无差异(P>； 0.0 5)。限制组胎肝中TLR2和TLR4mRNA的表达明显高于对照组(P< 和P<0.05)。此外，限制组髓系分化初级反应基因MYD88、肿瘤坏死因子受体相关因子6、核因子kappaB亚单位1、神经生长因子B抑制因子α、干扰素-β、转化生长因子-β、肿瘤坏死因子-α和IL-1β的表达水平在限制组显著上调(P<0.05)，TLR3的表达水平在限制组有升高趋势(P<0.05)，而TLR3的表达有升高的趋势(P =0.099)。而TLR2、TLR4、IκBα、磷酸化IκBα、磷酸化IκBα/TOTAL IκBα、TRIF和MyD88的蛋白水平并未受到母体限饲的影响(P >；0.05)。
BackgroundLiver has important immune function during fetal development and after birth. However, the effect of maternal malnutrition on immune function of the fetal liver is rarely reported. In this study, twelve pregnant goats (Xiangdong black goat, at d 45 of gestation) were assigned to the control group (fed 100% of nutritional requirements) and the restriction group (fed 60% of the intake of the control group) during gestation from d 55 to 100. Fetal goats were harvested at d 100 of gestation and immune indexes and amino acid profiles of the umbilical cord blood and liver Toll-like receptors (TLRs) signaling pathways were measured.ResultsMaternal body weight in the restriction group was lower than the control group (P< 0.05). Maternal feed intake restriction decreased (P <0.05) heart weight, heart index, alkaline phosphatase and serum amyloid protein A in the umbilical cord blood (UCB). Moreover, only histidine was decreased in the restricted group (P= 0.084), and there were no differences in other amino acids contents in the UCB between the two groups (P> 0.05). TheTLR2andTLR4mRNA expression in the fetal liver in the restriction group was greater (P <0.05) than that in the control group. Furthermore, the mRNA expression levels of myeloid differentiation primary response 88 (MyD88), TNF receptor associated factor 6, nuclear factor kappa B subunit 1, NFKB inhibitor alpha,IFN-β,TGF-β,TNF-αandIL-1βin the restricted group were upregulated (P <0.05), and the expression ofTLR3(P =0.099) tended to be higher in the restricted group. However, protein levels of TLR2, TLR4, IκBα, phosphorylated IκBα, phosphorylated IκBα/total IκBα, TRIF and MyD88 were not affected (P >0.05) by maternal intake restriction.ConclusionsThese results revealed that the restriction of maternal feed intake influenced the development of heart and hepatic protein synthesis at the acute phase of fetal goats and upregulated the mRNA expression of genes involved in MyD88-dependent signaling pathways and of target cytokines.