Conductance recording of ionic outflow from frog skin glands during nerve stimulation.
Conductance recording of ionic outflow from frog skin glands during nerve stimulation.
复制标题
神经刺激过程中青蛙皮腺离子流出的电导记录。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
C. Skoglund
中科院分区：
文献类型：
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作者：
L. Lang;E. Sjöberg;C. Skoglund
建立了一种连续记录蛙皮腺离子外流的方法，在给定的实验条件下，提供了一种简便的腺体分泌指标。从小牛身上取下一种神经皮肤制剂，这样皮肤的外部就形成了一个装有蒸馏水的小测试室的底部，而真皮一侧则在林格氏溶液中沐浴。在确定了发生可辨别腺体分泌的适当神经刺激参数后，在不同的时间段以10赫兹的频率刺激皮肤神经，并对测试室溶液中离子含量的变化进行化学对照分析。离子外流主要由Na+和Cl-等量组成，刺激过程中其他离子的外流可以忽略不计。通过施加到放置在测试室中的电导探头的高频场中的吸收变化来测量伴随的电导变化。根据电导变化测定的氯化钠流出量与化学分析得到的值符合得很好。连续的电导记录证明给出了腺体分泌物的数量和时间关系的近似信息，允许与例如皮肤电位的变化进行直接比较。
A method for continuous recording of the ionic efflux from frog skin glands has been developed which under given experimental conditions provides a convenient index on glandular secretion. A nerve skin preparation from the calf is mounted so that the outside of the skin forms the bottom of a small test compartment with distilled water while the corium side is bathing in Ringer's solution. After determination of the adequate nerve stimulus parameters for discernible gland secretion to occur, the skin nerve was stimulated at 10 Hz during varying periods and chemical control analyses performed of the changes in ionic content of the test compartment solution. The main ionic outflow consisted of Na+ and Cl- in equal amounts, the outflow of other ions during stimulation being negligible. The concomitant conductance changes were measured as variations of absorption in a high-frequency field applied to a conductance probe placed in the test chamber. Determinations of the NaCl outflow based on the conductance changes were in good agreement with the values obtained by chemical analysis. The continuous conductance recordings proved to give approximative information of the quantities and time relations of the glandular secretion, allowing a direct comparison with, e.g., skin potential changes.