Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis
Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis
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DOI：
10.1111/j.1574-6968.2009.01498.x
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发表时间：
2009-03-01
影响因子：
2.1
通讯作者：
Grubhoffer, Libor
中科院分区：
文献类型：
--
作者：
Rudenko, Nataliia;Golovchenko, Maryna;Grubhoffer, Libor
直到最近，三种螺旋体基因种被认为是欧洲莱姆疏螺旋体病 (LB) 的病原体：严格伯氏疏螺旋体、阿夫泽勒疏螺旋体和加氏疏螺旋体。然而，瓦莱疏螺旋体、卢西塔疏螺旋体、斯氏疏螺旋体和比塞氏疏螺旋体的 DNA 已在人类样本中检测到，或者从有 LB 症状的患者中分离出螺旋体。对地区医院收集的 12 份选定血清样本进行的分子分析证实，在有症状的疏螺旋体病或慢性疏螺旋体感染患者的单一和多重感染病例中存在比塞提伯氏疏螺旋体 DNA。患者体内存在比塞蒂伯氏杆菌作为单一菌株，这有力地支持了比塞蒂伯伯氏菌可能是该疾病的病原体这一事实。继斯洛文尼亚首次从人类来源的样本中分离出比塞蒂伯氏杆菌之后，继在捷克共和国心内膜炎和主动脉瓣狭窄患者的心脏瓣膜组织中检测到该物种之后，我们在这里提供了支持欧洲LB中伯氏杆菌参与的额外分子数据。
Until recently, three spirochete genospecies were considered to be the causative agents of Lyme borreliosis (LB) in Europe: Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. However, the DNA of Borrelia valaisiana, Borrelia lusitaniae, Borrelia spielmanii and Borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of LB. Molecular analysis of 12 selected serum samples collected in the regional hospital confirmed the presence of B. bissettii DNA in cases of single and multiple infection in patients with symptomatic borreliosis or chronic borrelial infection. The presence of B. bissettii as a single strain in patients provides strong support of the fact that B. bissettii might be a causative agent of the disease. After the first isolation of B. bissettii from the samples of human origin in Slovenia, following the detection of this species in cardiac valve tissue of the patient with endocarditis and aortic valve stenosis in the Czech Republic, here we present additional molecular data supporting the involvement of B. bissettii in LB in Europe.