In vitro and in vivo characterization of a novel H1N1/2009 influenza virus reassortant with an NS gene from a highly pathogenic H5N1 virus, isolated from a human
In vitro and in vivo characterization of a novel H1N1/2009 influenza virus reassortant with an NS gene from a highly pathogenic H5N1 virus, isolated from a human
复制标题
新型 H1N1/2009 流感病毒重配体的体外和体内表征，其 NS 基因来自从人类分离的高致病性 H5N1 病毒
DOI：
10.1007/s00705-017-3408-z
复制
发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Liu K
中科院分区：
文献类型：
--
作者：
Liu S;Zhang L;Yao Z;Xing L;Liu K
引起21世纪世纪第一次流感大流行的三重H1N1/2009甲型流感病毒通常与轻度疾病和相对较低的死亡率相关，与季节性流感病毒爆发的死亡率相当。人们越来越担心低死亡率H1N1/2009和其他高死亡率流感病毒之间可能发生重配。在这里，我们描述和表征了一种新的抗H1N1/2009流感病毒，从人体样本中分离，其中含有来自高致病性H5 N1病毒的NS基因。我们评估了获得的NS基因对病毒毒力的影响，在体外和体内，发现新的NS重配流感病毒复制以及在不同的细胞系和BALB/c小鼠的几个器官，没有事先适应，并诱导细胞因子失衡。因此，H1N1/2009病毒继续存在进一步重组的风险，因此，应加强系统监测，为下一次可能的大流行做好准备。
The triple-reassortant H1N1/2009 influenza A virus, which caused the first influenza pandemic of the 21st century, is generally associated with mild disease and a relatively low mortality rate comparable to that of seasonal influenza virus outbreaks. There is a growing concern about the potential for reassortment between the low-mortality H1N1/2009 and other high-mortality influenza viruses. Here, we describe and characterize a novel reassortant H1N1/2009 influenza virus, isolated from a human sample, that contained an NS gene from a highly pathogenic H5N1 virus. We evaluated the effect of the acquired NS gene on viral virulence both in vitro and in vivo and found that the novel NS-reassorted influenza virus replicated well in different cell lines and several organs of BALB/c mice without prior adaption and induced a cytokine imbalance. Therefore, there is a continued risk for further reassortment of the H1N1/2009 virus, and therefore, systematic surveillance should be enhanced to prepare for the next possible pandemic.
登录
查看更多内容
影响因子：
64.5
作者：
Fauci AS
通讯作者：
Fauci AS
结合诱导的本性非结构化转录因子的折叠。
影响因子：
4.3
作者：
Turjanski, Adrian Gustavo;Gutkind, J. Silvio;Best, Robert B.;Hummer, Gerhard
通讯作者：
Hummer, Gerhard
DOI：
10.1073/pnas.85.2.324
发表时间：
1988-01-01
影响因子：
11.1
作者：
KAWAOKA, Y;WEBSTER, RG
通讯作者：
WEBSTER, RG
DOI：
10.1016/j.bbrc.2014.04.132
发表时间：
2014-06-20
影响因子：
3.1
作者：
Kim, Jin Il;Hwang, Min-Woong;Park, Man-Seong
通讯作者：
Park, Man-Seong
影响因子：
64.8
作者：
Taubenberger, JK;Reid, AH;Fanning, TG
通讯作者：
Fanning, TG