Quantification of change in vocal fold tissue stiffness relative to depth of artificial damage.
Quantification of change in vocal fold tissue stiffness relative to depth of artificial damage.
复制标题
声带组织硬度相对于人工损伤深度的变化的量化。
DOI：
10.1080/14015439.2016.1221445
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Rohlfs AK
中科院分区：
文献类型：
--
作者：
Rohlfs AK
目的：量化人工损伤人声襞切除后生物力学特性的变化。方法：采用线性皮肤流变仪（LSR）对30例人尸体声带表面的剪切模量进行了一系列流变测量。组织样品最初在自然条件下进行测量，然后进行不同强度的热损伤。对每个声带进行组织学检查以确定人工改变的深度。测量的刚度变化与细胞损伤的深度相关。结果：声带损伤前剪切模量为537 ~ 1651 Pa（女性）和583 ~ 1193 Pa（男性）。随着固有层（LP）中间层损伤深度的增加，在对声带进行热损伤后，组织刚度持续增加（与原始值相比）。测量结果表明，当组织损伤深度从中间LP层向下延伸时，组织刚度增加。结论：体外实验证实了人声带组织在一定深度损伤后弹性特性的变化。未来，弹性声带组织改变的可重复性视觉测量可能使声外科医生能够从表面弹性的变化中推断上皮下损伤的程度。
Objectives:To quantify changes in the biomechanical properties of human excised vocal folds with defined artificial damage.Methods:The linear skin rheometer (LSR) was used to obtain a series of rheological measurements of shear modulus from the surface of 30 human cadaver vocal folds. The tissue samples were initially measured in a native condition and then following varying intensities of thermal damage. Histological examination of each vocal fold was used to determine the depth of artificial alteration. The measured changes in stiffness were correlated with the depth of cell damage.Results:For vocal folds in a pre-damage state the shear modulus values ranged from 537 Pa to 1,651 Pa (female) and from 583 Pa to 1,193 Pa (male). With increasing depth of damage from the intermediate layer of the lamina propria (LP), tissue stiffness increased consistently (compared with native values) following application of thermal damage to the vocal folds. The measurement showed an increase of tissue stiffness when the depth of tissue damage was extending from the intermediate LP layer downwards.Conclusions:Changes in the elastic characteristics of human vocal fold tissue following damage at defined depths were demonstrated in anin vitroexperiment. In future, reproduciblein vivomeasurements of elastic vocal fold tissue alterations may enable phonosurgeons to infer the extent of subepithelial damage from changes in surface elasticity.