Rapid and quantitative detection of blood Serratia marcescens by a real-time PCR assay:: Its clinical application and evaluation in a mouse infection model
Rapid and quantitative detection of blood Serratia marcescens by a real-time PCR assay:: Its clinical application and evaluation in a mouse infection model
复制标题
DOI：
10.1016/j.femsle.2005.05.041
复制
发表时间：
2005-07-15
影响因子：
2.1
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Iwaya, A;Nakagawa, S;Yamamoto, T
在日本，大规模医院内爆发的粘质沙雷氏菌败血症在48小时内的死亡率为20-60%。作为对策，建立了实时荧光定量PCR快速诊断方法。粘质败血症该试验确实检测到了S。临床血液样本中的粘质纤维素（约10（2）CFU ml（-1）），在疑似败血症病例中的频率为0.5%。在小鼠中，该测定提供了血液S的估计值。在不同感染阶段的粘质水平：即，在致死阶段为10（7）至10（8）CFU ml（-1（导致100%死亡），10（4）-10（5）CFU ml（-1），处于中度致死阶段（导致50%或更多的死亡），并且在轻度阶段< 103 CFU ml（-1）（导致100%存活），与实际CFU测量一致。血液细菌水平可能是反映败血症严重程度的重要临床标志物。同时检测S.粘质杆菌和碳青霉烯类耐药基因也得到了证实。(c)2005年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Larize-scale nosocomial outbreaks of Serratia marcescens septicaemia in Japan have had a fatality rate of 20-60% within 48 h. As a countermeasure, a real-time PCR assay was constructed for the rapid diagnosis of S. marcescens septicaemia. This assay indeed detected S. marcescens in clinical blood specimens (at ca. 10(2) CFU ml(-1)), at a frequency of 0.5% in suspected cases of septicaemia. In mice, the assay provided estimates of blood S. marcescens levels at various infectious stages: namely, 10(7) to 10(8) CFU ml(-1) at a fatal stage (resulting in 100% death), 10(4)-10(5) CFU ml(-1) at a moderately fatal stage (resulting in 50% or more death), and < 103 CFU ml(-1) at a mild stage (resulting in 100% survival), consistent with actual CFU measurements. Blood bacterial levels could be an important clinical marker that reflects the severity of septicaemia. The simultaneous detection of S. marcescens and the carbapenem resistance gene was also demonstrated. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.