The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis
The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis
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DOI：
10.1080/15592324.2017.1316442
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发表时间：
2017-01-01
影响因子：
2.9
通讯作者：
Yan, Jianbin
中科院分区：
文献类型：
--
作者：
Deng, Cuiyun;Ye, Haiyan;Yan, Jianbin
低温胁迫是限制作物产量的主要因素之一。植物反过来又进化出高度复杂的机制，包括改变生理和生化过程，以科普冷胁迫。先前的研究表明，CBF表达诱导因子1（ICE 1）是一种碱性螺旋-环-螺旋（bHLH）转录因子，直接结合并激活C-重复结合因子/脱水反应元件结合蛋白（CBF/DREB 1）的表达，以调节拟南芥的冷反应途径。然而，AtICE 1直向同源物在水稻中的功能在很大程度上是未知的。本研究发现水稻OsICE 1和OsICE 2与拟南芥AtICE 1具有高度保守的氨基酸序列。OsICE 1和OsICE 2在拟南芥中的过表达显著增强了拟南芥幼苗的耐冷性，提高了冷反应基因的表达。此外，我们发现OsICE 1和OsICE 2都与水稻冷适应所必需的单一DNA结合重复MYB转录因子OsMYBS 3发生物理相互作用，这表明OsICE 1/OsICE 2和OsMYBS 3可能通过特定的信号转导机制来协调水稻的耐冷性。这些结果表明，这2个OsICE基因与AtICE 1基因同源，在激活冷反应基因、调节植物耐冷性方面起着正向调节作用。
Cold stress is one of the major constraints for crop yield. Plants have in turn evolved highly sophisticated mechanisms involving altered physiologic and biochemical processes to cope with the cold stress. Previous studies have revealed that the INDUCER OF CBF EXPRESSION 1 (ICE1), a basic helix-loop-helix (bHLH) transcription factor, directly binds and activates the expression of C-Repeat Binding Factor/Dehydration-Responsive-Element-Binding protein (CBF/DREB1) to regulate the cold-response pathway in Arabidopsis thaliana. However, the function of AtICE1 orthologues in rice is largely unknown. Here we identified that OsICE1 and OsICE2 in rice shared highly conserved amino acid sequence with AtICE1 in Arabidopsis. Overexpression of OsICE1 and OsICE2 in Arabidopsis significantly enhanced the cold tolerance of Arabidopsis seedlings and improved the expression of cold-response genes. Furthermore, we showed that both OsICE1 and OsICE2 physically interact with OsMYBS3, a single DNA-binding repeat MYB transcription factor that is essential for cold adaptation in rice, suggesting that OsICE1/OsICE2 and OsMYBS3 probably act through specific signal transduction mechanisms to coordinate cold tolerance in rice. These results demonstrated that the 2 OsICEs are orthologues of AtICE1 and play positive regulators in activation of cold-response genes to regulate the cold tolerance.