Screening of wheat endophytes as biological control agents against Fusarium head blight using two different in vitro tests
Screening of wheat endophytes as biological control agents against Fusarium head blight using two different in vitro tests
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DOI：
10.1016/j.micres.2017.04.014
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发表时间：
2017-01-01
影响因子：
6.7
通讯作者：
Baillieul, Fabienne
中科院分区：
文献类型：
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作者：
Comby, Morgane;Gacoin, Marie;Baillieul, Fabienne
为了寻找防治小麦主要病害赤霉病（Fusarium head blight， FHB）的生物防治剂（bca），采用体外双培养法对86种小麦内部组织分离的微生物进行了鉴定，发现它们具有抑制禾枯镰刀菌（Fusarium graminearum）和枯枯镰刀菌（Fusarium culmorum）生长的能力。一组22株菌株对谷草镰刀菌的抑制率为30 ~ 51%，在全球范围内对谷草镰刀菌的抑制率为15 ~ 53%。通过离体小穗筛选进一步鉴定了3种尚未报道的抗镰刀菌菌株，即Phoma glomerata、Aureobasidium proteae和Sarocladium kiliense，这表明在小麦内生菌中寻找针对FHB的bcaa是有希望的。两种体外筛选方法对某些菌株的效果存在差异，因此寻找最合适的bca筛选方法至关重要。本研究指出了对小麦离体小穗试验的兴趣，该试验提供了内生菌株在目标植株上的潜在致病性、生长能力和功效等信息，然后再对其进行全株试验。
In order to find biological control agents (BCAs) for the management of Fusarium head blight (FHB), a major disease on wheat crops worldwide, 86 microorganisms isolated from inner tissues of wheat plants were discriminated for their ability to inhibit the growth of Fusarium graminearum and Fusarium culmorum by in vitro dual culture assays. A group of 22 strains appeared very effective to inhibit F. graminearum (inhibition of 30-51%) and they were also globally effective in controlling F. culmorum (inhibition of 15-53%). Further evaluation of a subselection of strains by screening on detached spikelets in vitro confirmed three species, namely Phoma glomerata, Aureobasidium proteae and Sarocladium kiliense, that have not yet been reported for their efficacy against Fusarium spp., indicating that looking for BCAs toward FHB among wheat endophytes proved to be promising. The efficacy of some strains turned out different between both in vitro screening approaches, raising the importance of finding the most appropriate screening approach for the search of BCAs. This study pointed out the interest of the test on detached wheat spikelets that provided information about a potential pathogenicity, the growth capacity and efficacy of the endophyte strains on the targeted plant, before testing them on whole plants.