Estrogen-dependent expression of the chicken very low density apolipoprotein II gene in serum-free cultures of LMH cells.
Estrogen-dependent expression of the chicken very low density apolipoprotein II gene in serum-free cultures of LMH cells.
复制标题
LMH 细胞无血清培养物中鸡极低密度载脂蛋白 II 基因的雌激素依赖性表达。
DOI：
10.1007/bf02634041
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Evans,MI
中科院分区：
文献类型：
--
作者：
Berkowitz,EA;Evans,MI
雌激素敏感的来航鸡M株鸡肝癌(LMH)细胞系为研究禽类基因的雌激素依赖性、肝脏特异性表达提供了一个模型系统。已经建立了无血清培养条件，允许载脂蛋白B、极低密度载脂蛋白II(ApoVLDLII)、血清白蛋白和转铁蛋白在Northern印迹分析中检测到的水平表达。在无血清培养的LMH细胞中，雌激素以适当的时间和剂量依赖的方式调节apoVLDLII基因的表达。在无血清培养条件下，apoVLDLII基因的表达水平是含10%血清培养条件下的100倍以上。在含10%血清的LMH细胞中，雌激素受体的水平约为每细胞2000个受体，而在无血清培养中，每个细胞的雌激素受体水平约为1000个。将雌激素受体DNA导入这些细胞后，在无血清培养条件下，apoVLDLII基因的表达较对照组明显降低。这些结果表明，当LMH细胞在无血清培养时，雌激素受体不是apoVLDLII基因表达的限制因素。
The estrogen-responsive Leghorn strain M chicken hepatoma (LMH) cell line provides a model system for studying the estrogen-dependent, liver-specific expression of avian genes. Serum-free culture conditions have been established that allow expression of apolipoprotein B, very low density apolipoprotein II (apoVLDLII), serum albumin, and transferrin at levels detectable by Northern blot analysis. Regulation of apoVLDLII mRNA by estrogen occurred in an appropriate time-and dose-dependent manner in serum-free cultures of the LMH cells. The expression of apoVLDLII mRNA in serum-free culture was at least 100-fold higher than that expressed in cultures containing 10% serum. The level of estrogen receptors in LMH cells cultured with 10% serum was approximately 2000 receptors per cell, and in serum-free culture approximately 1000 receptors per cell. When these cells were transfected with estrogen receptor DNA and cultured in serum-free medium, apoVLDLII mRNA was decreased relative to that expressed in cells transfected with a control plasmid. These results indicate that when the LMH cells are cultured without serum, estrogen receptors are not the limiting factor for the expression of the apoVLDLII gene.