Filamins but Not Janus Kinases Are Substrates of the ASB2α Cullin-Ring E3 Ubiquitin Ligase in Hematopoietic Cells
Filamins but Not Janus Kinases Are Substrates of the ASB2α Cullin-Ring E3 Ubiquitin Ligase in Hematopoietic Cells
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DOI：
10.1371/journal.pone.0043798
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发表时间：
2012-08-20
期刊：
影响因子：
3.7
通讯作者：
Lutz, Pierre G.
中科院分区：
文献类型：
--
作者：
Lamsoul, Isabelle;Erard, Monique;Lutz, Pierre G.
The ASB2 alpha protein is the specificity subunit of an E3 ubiquitin ligase complex involved in hematopoietic differentiation and is proposed to exert its effects by regulating the turnover of specific proteins. Three ASB2 alpha substrates have been described so far: the actin-binding protein filamins, the Mixed Lineage Leukemia protein, and the Janus kinases 2 and 3. To determine the degradation of which substrate drives ASB2 alpha biological effects is crucial for the understanding of ASB2 alpha functions in hematopoiesis. Here, we show that neither endogenous nor exogenously expressed ASB2 alpha induces degradation of JAK proteins in hematopoietic cells. Furthermore, we performed molecular modeling to generate the first structural model of an E3 ubiquitin ligase complex of an ASB protein bound to one of its substrates.
