A convenient radiometric assay for hyaluronan.
A convenient radiometric assay for hyaluronan.
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一种方便的透明质酸放射测定法。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
A. Tengblad
中科院分区：
文献类型：
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作者：
R. Brandt;E. Hedlöf;I. Asman;A. Bucht;A. Tengblad
从牛软骨中分离出透明质酸结合蛋白(HABP)，用于测定血清中透明质酸(HA)的放射分析。待分析样品与加碘的HABP预孵化。60分钟后，溶液中未与透明质酸结合的蛋白质在45分钟的孵育中在HA-Sepharose上被回收。透明质酸的测定范围为10~1000微克/L，进样量为100微升，批内和批间变异系数分别小于10%和小于10%，检出限为4微克/L。
Hyaluronan-binding proteins (HABP) isolated from bovine cartilage have been utilized in a radiometric assay for determination of hyaluronan (hyaluronic acid; HA) in serum. The samples to be analysed are preincubated with iodinated HABP. After 60 minutes the protein that is not bound to hyaluronan, in the solution, is recovered on HA-Sepharose in a 45-min incubation. Hyaluronan can be determined in the range from 10 to 1,000 micrograms/l. The sample size is 100 microliter and the intra-assay and inter-assay coefficients of variations are less than 10% and less than 10%, respectively for samples containing hyaluronan concentrations in the range 10-200 micrograms/l. Detection limit is 4 micrograms/l.