Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: a novel strategy to promote bone regeneration
Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: a novel strategy to promote bone regeneration
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将间充质干细胞片与富含血小板的血浆凝胶/磷酸钙颗粒相结合：促进骨再生的新策略
DOI：
10.1186/s13287-015-0256-1
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发表时间：
2015-12-21
影响因子：
7.5
通讯作者：
Huang, Zhongming
中科院分区：
文献类型：
--
作者：
Qi, Yiying;Niu, Lie;Huang, Zhongming
背景：促进骨再生是骨缺损成功修复的关键。本研究旨在探讨骨髓间充质干细胞（BMSC）片与富血小板血浆（PRP）凝胶/磷酸钙颗粒复合后能否促进大鼠股骨骨缺损的成骨。在体内，将36个2.5 x 5 mm的骨缺损随机分组，并用BMSC/PRP凝胶、磷酸钙颗粒、PRP凝胶/磷酸钙颗粒、BMSC片层/磷酸钙颗粒、BMSC片层/PRP凝胶/磷酸钙颗粒处理，或不处理（n = 6/组）。另外15个骨缺损用氯甲基-苯甲酰氨基二烷基羰花青（CM-Dil）标记的BMSC片/PRP凝胶/磷酸钙颗粒处理，并使用小动物体内荧光成像系统在术后1天、3天、7天、2周和4周追踪植入的BMSC。经PRP和磷酸钙颗粒处理的BMSCs或BMSC片的I型胶原和骨钙素基因表达显著高于经磷酸钙颗粒处理的BMSCs或BMSC片或对照组（P < 0.05）。PRP可促进BMSC和BMSC片中III型胶原和腱调节蛋白的基因表达。VEGF、I型胶原和骨钙素基因在体外培养的BMSC中表达明显高于体外培养的BMSC（P < 0.05）。此外，茜素红染色定量，ALP定量和钙黄绿素蓝荧光显示了PRP和磷酸钙颗粒处理的BMSCs的成骨潜力。植入的BMSCs在术后1天，3天，7天，2周和4周通过小动物体内荧光成像系统可检测到，并通过共聚焦显微镜观察到骨缺损区。在植入后4周，与BMSC片/PRP凝胶/磷酸钙颗粒治疗的缺陷表现出显着更多的骨形成比其他五groups.Conclusions：将BMSC片纳入PRP凝胶/磷酸钙颗粒大大促进骨再生。这些BMSC片层和组织工程策略为临床促进骨缺损修复提供了治疗机会。
Background: Promotion of bone regeneration is important for successful repair of bony defects. This study aimed to investigate whether combining bone marrow-derived mesenchymal stem cell (BMSC) sheets with platelet-rich plasma (PRP) gel/calcium phosphate particles could promote bone formation in the femoral bone defects of rats.Methods: The proliferation and differentiation of BMSCs or BMSC sheets cultured with calcium phosphate particles and/or PRP were investigated in in vitro. In vivo, 36 2.5 x 5 mm bone defects were randomly divided into groups and treated with either BMSCs/PRP gel, calcium phosphate particles, PRP gel/calcium phosphate particles, a BMSC sheet/calcium phosphate particles, a BMSC sheet/PRP gel/calcium phosphate particles, or were left untreated (n = 6/group). A further 15 bone defects were treated with chloromethyl-benzamidodialkylcarbocyanine (CM-Dil)-labelled BMSC sheet/PRP gel/calcium phosphate particles and observed using a small animal in vivo fluorescence imaging system to trace the implanted BMSCs at 1 day, 3 days, 7 days, 2 weeks, and 4 weeks after surgery.Results: The expression of collagen type I and osteocalcin genes of BMSCs or BMSC sheets treated with PRP and calcium phosphate particles was significantly higher than that of BMSCs or BMSC sheets treated with calcium phosphate particles or the controls (P < 0.05). PRP can promote gene expression of collagen III and tenomodulin by BMSCs and in BMSC sheets. The VEGF, collagen I and osteocalcin gene expression levels were higher in the BMSC sheet than in cultured BMSCs (P < 0.05). Moreover, alizarin red staining quantification, ALP quantification and calcein blue fluorescence showed the osteogenic potential of BMSCs treated with PRP and calcium phosphate particles The implanted BMSCs were detectable at 1 day, 3 days, 7 days, 2 weeks and 4 weeks after surgery by a small animal in vivo fluorescence imaging system and were visualized in the defect zones by confocal microscopy. At 4 weeks after implantation, the defects treated with the BMSC sheet/PRP gel/calcium phosphate particles showed significantly more bone formation than the other five groups.Conclusions: Incorporation of an BMSC sheet into the PRP gel/calcium phosphate particles greatly promoted bone regeneration. These BMSC sheet and tissue engineering strategies offer therapeutic opportunities for promoting bone defect repair clinically.