Tissue tropism and neuroinvasion of West Nile virus do not differ for two mouse strains with different survival rates
Tissue tropism and neuroinvasion of West Nile virus do not differ for two mouse strains with different survival rates
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DOI：
10.1016/j.virol.2007.06.033
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发表时间：
2007-11-25
期刊：
影响因子：
3.7
通讯作者：
Bernard, Kristen A.
中科院分区：
文献类型：
--
作者：
Brown, Ashley N.;Kent, Kim A.;Bernard, Kristen A.
西尼罗河病毒（WNV）是一种蚊媒黄病毒，感染人类和其他动物的中枢神经系统。在本研究中，我们发现C3 H/HeN（M）小鼠比C57 BL/6（B6）小鼠表现出更高的发病率和死亡率。我们比较了急性病毒感染期间C3 H和B6小鼠的组织嗜性、病毒复制和动力学。在多个组织中检测到WNV，包括皮肤、十二指肠和胰腺等新部位，但两种毒株的嗜性相同。此外，病毒载量和传播动力学在菌株之间没有实质性差异。神经侵袭发生在接种后第3天，在嗅球和脊髓中早期检测到两种菌株，表明西尼罗河病毒神经侵袭在特定部位。此外，CNS中的神经侵袭和病毒载量不能预测疾病的结果。我们的数据表明，C3 H和136小鼠之间的发病率和死亡率的差异是不是由于在急性西尼罗河病毒感染的嗜性，病毒载量或动力学的差异。(c)2007年爱思唯尔公司All rights reserved.
West Nile virus (WNV) is a mosquito-borne flavivirus that infects the central nervous system of humans and other animals. In this study, we found that C3H/HeN (M) mice exhibited a higher morbidity and mortality than C57BL/6 (B6) mice. We compared tissue tropism, viral replication and kinetics for C3H and B6 mice during acute viral infection. WNV was detected in multiple tissues, including novel sites such as the skin, duodenum and pancreas, but the tropism was identical for the two strains. Additionally, viral load and kinetics of spread did not differ substantially between strains. Neuroinvasion occurred in both strains by day 3 post-inoculation with early detection in the olfactory bulbs and spinal cord, suggesting that WNV neuroinvades at specific sites. Furthermore, neuroinvasion and viral load in the CNS did not predict disease outcome. Our data suggest that the disparities in morbidity and mortality between C3H and 136 mice are not due to differences in tropism, viral load or kinetics during acute WNV infection. (c) 2007 Elsevier Inc. All rights reserved.