Comparative proteomic profiling in squamous cell carcinoma of the uterine cervix
Comparative proteomic profiling in squamous cell carcinoma of the uterine cervix
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DOI：
10.1002/prca.201000077
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发表时间：
2011-04-01
影响因子：
2
通讯作者：
Sugino, Norihiro
中科院分区：
文献类型：
--
作者：
Fukushima, Chikako;Murakami, Akihiro;Sugino, Norihiro
目的：为寻找新的宫颈鳞癌早期诊断和分子靶向治疗的生物标志物，对5例正常宫颈组织和5例宫颈鳞癌组织进行蛋白质组学分析。实验设计：对5例正常宫颈组织和5例宫颈鳞癌组织中的蛋白质进行双向电泳（2-DE）和液相色谱-串联质谱（LC-MS/MS）分析。27个蛋白质斑点增加，其中5个通过LC-MS/MS鉴定为细胞角蛋白-19，HSP 70，HSP 27，甘油醛-3-磷酸脱氢酶和转胶蛋白-2。HSP 70、HSP 27和transgelin-2的上调通过Western印迹法检测培养的SCC细胞系、10个额外的正常组织、10个额外的SCC组织和3个配对的SCC组织及其相应的非癌组织的样品。HSP 70在鳞癌组织中的阳性表达率高于正常组织。Transgelin-2仅在SCC组织中观察到，而且在早期SCC组织中也观察到。结论和临床意义：本研究通过蛋白质组学分析成功鉴定了宫颈SCC中三种上调的蛋白质，并表明这些蛋白质可能是新的候选生物标志物，用于早期诊断SCC和分子靶向治疗。
Purpose: Proteomic profiling of human uterine cervical squamous cell carcinoma (SCC) tissues was performed to find new biomarkers for the early diagnosis and molecular target therapies.Experimental design: Proteins extracted from five of normal tissues and five of the SCC tissues were subjected to 2-DE followed by LC-MS/MS.Results: Twenty-seven of the protein spots were increased, and five of them were identified by LC-MS/MS to be cytokeratin-19, HSP70, HSP27, glyceraldehyde-3-phosphate dehydrogenase, and transgellin-2. The upregulation of HSP70, HSP27, and transgelin-2 was examined by Western blot of cultured SCC cell lines, ten additional normal tissues, ten additional SCC tissues, and three paired samples of the SCC tissue and its corresponding noncancerous tissue. The positive rate of HSP70 expression was higher in the SCC tissues than in normal tissues. Transgelin-2 was observed only in the SCC tissues, and also those in the early stage.Conclusions and clinical relevance: The present study successfully identified three upregulated proteins in the uterine cervical SCC by proteomic analyses, and suggested that these proteins could be the candidates for new biomarkers for early diagnosis of SCC and molecular target therapies.