ScMT2-1-3, a metallothionein gene of sugarcane, plays an important role in the regulation of heavy metal tolerance/accumulation.
ScMT2-1-3, a metallothionein gene of sugarcane, plays an important role in the regulation of heavy metal tolerance/accumulation.
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DOI：
10.1155/2013/904769
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发表时间：
2013
影响因子：
--
通讯作者：
Que Y
中科院分区：
文献类型：
--
作者：
Guo J;Xu L;Su Y;Wang H;Gao S;Xu J;Que Y
植物金属硫蛋白(MTs)是一种富含半胱氨酸、低分子量的金属结合蛋白，在解毒、金属离子动态平衡和金属运输调节等方面发挥着重要作用。本研究从甘蔗中克隆到一个新的金属硫蛋白基因，命名为ScMT2-1-3(GenBank登录号JQ627644)。ScMT2-1-3全长700 ，含一个240 的开放阅读框，编码79个氨基酸残基。成功地在大肠杆菌系统中表达了His标记的ScMT2-1-3蛋白，提高了宿主细胞对Cd~(2+)、Cu~(2+)、聚乙二醇和氯化钠的耐受性。ScMT2-1-3在Cu2+胁迫下表达上调，而在Cd+胁迫下表达下调。实时荧光定量聚合酶链式反应显示，ScMT2-1-3在芽和根中的表达水平分别是茎和叶中的14倍以上。因此，无论是大肠杆菌实验还是甘蔗试管苗实验都表明ScMT2-1-3与细胞内铜的解毒和储存密切相关，但其在甘蔗细胞镉解毒和储存中的作用机制还有待进一步的验证，尽管其表达的蛋白可以明显提高宿主大肠杆菌对Cd~(2+)的耐受性。因此，ScMT2-1-3为阐明MTS诱导的植物重金属耐性/积累的分子机制和培育甘蔗植物修复品种提供了一个新的候选基因。
Plant metallothioneins (MTs), which are cysteine-rich, low-molecular-weight, and metal-binding proteins, play important roles in detoxification, metal ion homeostasis, and metal transport adjustment. In this study, a novel metallothionein gene, designated as ScMT2-1-3 (GenBank Accession number JQ627644), was identified from sugarcane. ScMT2-1-3 was 700 bp long, including a 240 bp open reading frame (ORF) encoding 79 amino acid residues. A His-tagged ScMT2-1-3 protein was successfully expressed in Escherichia coli system which had increased the host cell's tolerance to Cd2+, Cu2+, PEG, and NaCl. The expression of ScMT2-1-3 was upregulated under Cu2+ stress but downregulated under Cd2+ stress. Real-time qPCR demonstrated that the expression levels of ScMT2-1-3 in bud and root were over 14 times higher than those in stem and leaf, respectively. Thus, both the E. coli assay and sugarcane plantlets assay suggested that ScMT2-1-3 is significantly involved in the copper detoxification and storage in the cell, but its functional mechanism in cadmium detoxification and storage in sugarcane cells needs more testification though its expressed protein could obviously increase the host E. coli cell's tolerance to Cd2+. ScMT2-1-3 constitutes thus a new interesting candidate for elucidating the molecular mechanisms of MTs-implied plant heavy metal tolerance/accumulation and for developing sugarcane phytoremediator varieties.
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影响因子：
5.1
作者：
Hsieh, HM;Liu, WK;Huang, PC
通讯作者：
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作者：
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