AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress.
AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress.
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DOI：
10.5483/bmbrep.2007.40.6.1083
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发表时间：
2007-11
期刊：
影响因子：
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通讯作者：
N. Chen;Xiulin Zhang;P. Wei;Qi-jun Chen;Fei Ren;Jia Chen;Xuecheng Wang
中科院分区：
文献类型：
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作者：
N. Chen;Xiulin Zhang;P. Wei;Qi-jun Chen;Fei Ren;Jia Chen;Xuecheng Wang
HAP复合物存在于许多真核生物中。HAP识别存在于30%的真核基因的启动子中的CCAAT盒。HAP复合物由HAP 2、HAP 3和HAP 5三个亚基组成。在本文中，我们报告的AtHAP 3b基因的生物学功能，编码的HAP 3亚基之一，在拟南芥。与野生型相比，突变体hap 3b-1和hap 3b-2在长日照条件下开花时间延迟。此外，FT的转录水平在突变体中比在野生型植物中低得多。这些结果表明AtHAP 3b可能通过调控FT的表达来调控拟南芥的开花时间。在随后的研究中，发现AtHAP 3b是由渗透胁迫诱导的。在渗透胁迫条件下，hap 3b-1和hap 3b-2突变体开花明显晚于野生型植物。这些结果表明AtHAP 3b基因在拟南芥渗透胁迫下的降低调控中起着更重要的作用。
The HAP complex has been found in many eukaryotic organisms. HAP recognizes the CCAAT box present in the promoters of 30% of all eukaryotic genes. The HAP complex consists of three subunits--HAP2, HAP3 and HAP5. In this paper, we report the biological function of the AtHAP3b gene that encodes one of the HAP3 subunits in Arabidopsis. Compared with wild-type plants, hap3b-1 and hap3b-2 mutants exhibited a delayed flowering time under long-day photoperiod conditions. Moreover, the transcription levels of FT were substantially lower in the mutants than in the wild-type plants. These results imply that AtHAP3b may function in the control of flowering time by regulating the expression of FT in Arabidopsis. In a subsequent study, AtHAP3b was found to be induced by osmotic stress. Under osmotic stress conditions, the hap3b-1 and hap3b-2 mutants flowered considerably later than the wild-type plants. These results suggest that the AtHAP3b gene plays more important roles in the control of lowering under osmotic stress in Arabidopsis.